The antenna of far-red absorbing cyanobacteria increases both absorption and quantum efficiency of Photosystem II

• Published in: Nature communications Vol. 13; no. 1; p. 3562
• Main Authors: Mascoli, Vincenzo, Bhatti, Ahmad Farhan, Bersanini, Luca, van Amerongen, Herbert, Croce, Roberta
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.06.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 140/125; 631/449/1734/2076; 631/449/1734/2077; 631/57/2267; Absorption; Absorption cross sections; Antennas; Charge efficiency; Chlorophyll; Cyanobacteria; Energy conversion; Fluorescence; Fluorescence spectroscopy; Humanities and Social Sciences; multidisciplinary; Photochemistry; Photons; Photosynthesis; Photosystem II; Phycobiliproteins; Quantum efficiency; Science; Science (multidisciplinary); Separation
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-022-31099-5

Cyanobacteria carry out photosynthetic light-energy conversion using phycobiliproteins for light harvesting and the chlorophyll-rich photosystems for photochemistry. While most cyanobacteria only absorb visible photons, some of them can acclimate to harvest far-red light (FRL, 700–800 nm) by integrating chlorophyll f and d in their photosystems and producing red-shifted allophycocyanin. Chlorophyll f insertion enables the photosystems to use FRL but slows down charge separation, reducing photosynthetic efficiency. Here we demonstrate with time-resolved fluorescence spectroscopy that on average charge separation in chlorophyll- f -containing Photosystem II becomes faster in the presence of red-shifted allophycocyanin antennas. This is different from all known photosynthetic systems, where additional light-harvesting complexes increase the overall absorption cross section but slow down charge separation. This remarkable property can be explained with the available structural and spectroscopic information. The unique design is probably important for these cyanobacteria to efficiently switch between visible and far-red light. Some cyanobacteria acclimate to far-red light by integrating chlorophyll f into their photosystems. Additional chlorophylls typically slow down charge separation but here the authors show that charge separation in chlorophyll-f-containing Photosystem II is faster in the presence of red-shifted allophycocyanin antennas.