Expression of genes encoding smooth muscle contractile proteins in vaginal tissue of women with and without pelvic organ prolapse
Aims We hypothesize that the expression of genes encoding vaginal smooth muscle (SM) contractile proteins is altered in patients with pelvic organ prolapse (POP) and is influenced by age and menopausal status. We aim to analyze the expression of SM‐myosin heavy chain (MHY11), caldesmon (CALD1), SM g...
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Published in | Neurourology and urodynamics Vol. 31; no. 1; pp. 109 - 114 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.01.2012
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Subjects | |
Online Access | Get full text |
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Summary: | Aims
We hypothesize that the expression of genes encoding vaginal smooth muscle (SM) contractile proteins is altered in patients with pelvic organ prolapse (POP) and is influenced by age and menopausal status. We aim to analyze the expression of SM‐myosin heavy chain (MHY11), caldesmon (CALD1), SM gamma‐actin (ACTG2), and tropomyosin (TPM1), in premenopausal and postmenopausal women with advanced POP and asymptomatic controls.
Methods
During total hysterectomy we collected anterior vaginal wall biopsy samples from 55 women, 37 premenopausal (23 patients and 14 controls), and 18 postmenopausal women (13 patients and 5 controls). Total mRNA from the tissues was quantified by real‐time RT‐PCR.
Results
MHY11 gene expression was down‐regulated in premenopausal POP patients compared to premenopausal controls (fivefold, P = 0.002). In the postmenopausal groups, we observed a sixfold increase in the CALD1 gene expression in POP patients compared to asymptomatic controls (P = 0.03). The gene expression of CALD1, ACTG2, and TPM1 was significantly down‐regulated in vaginal tissue of healthy women after menopause (P < 0.05).
Conclusion
Dysregulation of the vaginal SM content in POP patients involves alteration of different cellular pathways according to age and menopausal status. Neurourol. Urodynam. 31:109–114, 2012. © 2011 Wiley Periodicals, Inc. |
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Bibliography: | ArticleID:NAU21175 Conflict of interest: The authors declare no conflict of interest. Heinz Koelbl led the review process. Data presented at the 35th Annual Meeting of the International Urogynecologic Association (IUGA), in August 25, 2010, Toronto, ON, CA. istex:49F3A76749063222AF7D05C02B16F27F7926DDA9 ark:/67375/WNG-W4F0C79Q-0 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0733-2467 1520-6777 |
DOI: | 10.1002/nau.21175 |