Experimental and Mouse-Specific Computational Models of the Fbln4SMKO Mouse to Identify Potential Biomarkers for Ascending Thoracic Aortic Aneurysm

• Published in: Cardiovascular engineering and technology Vol. 13; no. 4; pp. 558 - 572
• Main Authors: Bazzi, Marisa S., Balouchzadeh, Ramin, Pavey, Shawn N., Quirk, James D., Yanagisawa, Hiromi, Vedula, Vijay, Wagenseil, Jessica E., Barocas, Victor H.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.08.2022; Springer Nature B.V
• Subjects: Aorta; Aortic aneurysms; Biomarkers; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedical Engineering/Biotechnology; Biomedicine; Cardiology; Correlation; Interaction models; Life span; Mouse devices; Original Article; Wall shear stresses; Biomechanics; Fluid–structure interaction; Biomarker; Aneurysm
• ISSN: 1869-408X; 1869-4098; 1869-4098
• DOI: 10.1007/s13239-021-00600-4

Purpose To use computational methods to explore geometric, mechanical, and fluidic biomarkers that could correlate with mouse lifespan in the Fbln4 SMKO mouse. Mouse lifespan was used as a surrogate for risk of a severe cardiovascular event in cases of ascending thoracic aortic aneurysm. Methods Image-based, mouse-specific fluid-structure-interaction models were developed for Fbln4 SMKO mice (n = 10) at ages two and six months. The results of the simulations were used to quantify potential biofluidic biomarkers, complementing the geometrical biomarkers obtained directly from the images. Results Comparing the different geometrical and biofluidic biomarkers to the mouse lifespan, it was found that mean oscillatory shear index (OSI min ) and minimum time-averaged wall shear stress (TAWSS min ) at six months showed the largest correlation with lifespan (r 2 = 0.70, 0.56), with both correlations being positive (i.e., mice with high OSI mean and high TAWSS min tended to live longer). When change between two and six months was considered, the change in TAWSS min showed a much stronger correlation than OSI mean (r 2 = 0.75 vs. 0.24), and the correlation was negative (i.e., mice with increasing TAWSS min over this period tended to live less long). Conclusion The results highlight potential biomarkers of ATAA outcomes that can be obtained through noninvasive imaging and computational simulations, and they illustrate the potential synergy between small-animal and computational models.