Analysis of the interaction of 16S rRNA and cytoplasmic membrane with the C‐terminal part of the Streptococcus pneumoniae Era GTPase

• Published in: European journal of biochemistry Vol. 268; no. 21; pp. 5570 - 5577
• Main Authors: Hang, Julie Qi, Meier, Timothy I., Zhao, Genshi
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.11.2001
• Subjects: 16S rRNA; Amino Acid Sequence; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Base Sequence; Binding Sites; Cell Division - genetics; Conserved Sequence; Cytoplasm - metabolism; Era GTPase; Escherichia coli - genetics; Escherichia coli Proteins; Genetic Complementation Test; GTP Phosphohydrolases - genetics; GTP Phosphohydrolases - metabolism; GTP-Binding Proteins - genetics; GTP-Binding Proteins - metabolism; Intracellular Membranes - metabolism; KH domain; Membrane Proteins - genetics; Membrane Proteins - metabolism; membrane‐binding; Molecular Sequence Data; Mutation; RNA, Bacterial - metabolism; RNA, Ribosomal, 16S - metabolism; RNA-Binding Proteins; RNA‐binding; Streptococcus pneumoniae - metabolism
• ISSN: 0014-2956; 1432-1033
• DOI: 10.1046/j.1432-1033.2001.02493.x

Era, an essential GTPase, plays a regulatory role in several cellular processes. The Era protein of Streptococcus pneumoniae has recently been shown to bind to 16S rRNA and the cytoplasmic membrane. However, exact locations of Era responsible for RNA‐ and membrane‐binding were unknown. To identify the regions in Era that interact with the RNA and membrane, the C‐terminal part of S. pneumoniae Era was systematically deleted while the N‐terminal part, responsible for the GTPase activity of the protein, was kept intact. The resulting truncated Era proteins were purified and characterized. The C‐terminal deletion of 9 or 19 amino‐acid residues did not affect 16S rRNA‐binding activity while further deletions of the C‐terminus (29–114 amino‐acid residues) abolished the activity. These results indicate that the integrity of the putative KH domain of Era, spanning the amino‐acid residues between ≈ 22–83 from the C‐terminus, is required for 16S rRNA‐binding. Furthermore, the Era proteins with a deletion up to 45 residues from the C‐terminus retained membrane‐binding activity, but longer deletions significantly reduced the activity. These results indicate that part of the putative KH domain is also required for membrane‐binding. Thus, these results indicate for the first time that the regions critical for the membrane‐ and 16S rRNA‐binding activities of Era overlap. The era gene with a deletion of 9 or 19 codons from its 3′ terminus complemented an Escherishia coli mutant strain deficient in Era production whereas the genes with longer deletions failed to do so, thereby indicating that the KH domain is essential for Era function. Taken together, the results of this study indicate that the putative KH domain is required for 16S rRNA‐binding activity and that part of the KH domain is also required for membrane‐binding activity. The results also suggest that the interaction between Era and 16S rRNA is essential for bacterial growth.