Elicitation with methyl-jasmonate stimulates peruvoside production in cell suspension cultures of Thevetia peruviana

• Published in: In vitro cellular & developmental biology. Plant Vol. 46; no. 3; pp. 233 - 238
• Main Authors: Zabala, Mario Arias, Angarita, Mónica, Restrepo, Juan M, Caicedo, Luis A, Perea, Margarita
• Format: Journal Article
• Language: English
• Published: New York New York : Springer-Verlag 01.06.2010; Springer; Springer-Verlag; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Cell Biology; Cell culture techniques; Cell growth; Cellular metabolism; Chemical suspensions; Culture media; Cultured cells; Developmental Biology; Growth kinetics; Kinetics; Latin American culture; Life Sciences; Metabolites; Microbiology; Plant Breeding/Biotechnology; Plant cells; Plant Genetics and Genomics; Plant Sciences; Plants; SECONDARY METABOLISM; Secondary Metabollism; Studies; Peruvoside; Culture media; Elicitation; Plant cell culture; Methyl-jasmonate
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/s11627-009-9249-z

Thevetia peruviana is a small tree that produces several compounds with pharmaceutical application, among which peruvoside could be highlighted. However, these compounds are produced in low concentration in the plant, making it important to develop strategies such as plant cell culture and elicitation to obtain higher quantities of the desired product. In this work, cell suspension cultures of T. peruviana were established in four different culture media: Murashige-Skoog (MS), half Murashige-Skoog (half MS), Schenk-Hildebrandt (SH), and Gamborg (B5) to study their effect on cell growth. Cell growth kinetics were studied in SH medium, and the extracellular peruvoside production during the culture time was determined. The best culture medium for the establishment of cell suspension cultures was MS with a growth index of 3.17 ± 0.2 g g⁻¹ inoculum. The cell growth kinetics showed the four characteristic growth phases of a cell culture (lag, exponential, stationary, and death), and during none of these phases was it possible to observe peruvoside production. The elicitor effect of methyl-jasmonate (MeJ) was studied in cell suspension cultures established in SH medium. The effect of MeJ concentration and the time in which it should be applied were determined. The best results were obtained at a concentration of 100 mg l⁻¹ of MeJ applied at the beginning of the culture, which induced a peruvoside production of 8.93 mg l⁻¹ medium. The current results are the first report of an in vitro peruvoside production system.