Ubiquitin gene expression: response to environmental changes

It has previously been shown that the yeast ubiquitin genes UBI1, 2 and 3 are strongly expressed during the log-phase of batch culture growth, whereas the UBI4 gene is weakly expressed. We found that heat shock, treatment with DNA-damaging agents, starvation, and the feeding of starved cells all tra...

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Bibliographic Details
Published inCurrent genetics Vol. 20; no. 1-2; p. 17
Main Authors Fraser, J. (Concordia Univ., Montreal (Canada). Dept. of Biology), Luu, H.A, Neculcea, J, Thomas, D.Y, Storms, R.K
Format Journal Article
LanguageEnglish
Published United States 01.01.1991
Subjects
4-Nitroquinoline-1-oxide - pharmacology
ADAPTACION
ADAPTATION
Adaptation, Biological - genetics
Base Sequence
Cell Cycle
Culture Media
Deoxyribonucleotides
ENVIRONMENTAL FACTORS
EXPRESION GENICA
EXPRESSION DES GENES
FACTEUR DU MILIEU
FACTORES AMBIENTALES
GENE EXPRESSION
Gene Expression Regulation, Fungal
Genes, Fungal
Hot Temperature
METABOLISM
METABOLISME
METABOLISMO
Methyl Methanesulfonate - pharmacology
Molecular Sequence Data
Mutation
PROTEINAS
PROTEINE
PROTEINS
Regulation
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - drug effects
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Ubiquitin
Ubiquitins - genetics
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Summary:It has previously been shown that the yeast ubiquitin genes UBI1, 2 and 3 are strongly expressed during the log-phase of batch culture growth, whereas the UBI4 gene is weakly expressed. We found that heat shock, treatment with DNA-damaging agents, starvation, and the feeding of starved cells all transiently induced UBI4. These results suggest that UBI4 is induced whenever a change in culture conditions dictates a dramatic shift in cellular metabolism, and that UBI4 expression returns to lower levels once cellular metabolism has adapted to the new conditions. In contrast, all of the treatments tested, except starvation, transiently repressed the UBI1, 2 and 3 genes. Although starvation also repressed UBI1, 2 and 3 its effect was not transient, and expression only recovered upon the addition of fresh media. These results, together with others presented here, suggest that high levels of UBI1, 2 and 3 expression are dependant upon ongoing cell growth, and that treatments which slow or stop growth repress their expression.
Bibliography:F30
93B0330
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00312760