Inhibition of experimental bone resorption and osteoclast formation and survival by 2-aminoethanesulphonic acid

• Published in: Archives of oral biology Vol. 44; no. 9; pp. 711 - 719
• Main Authors: Koide, Masanori, Okahashi, Nobuo, Tanaka, Rumi, Kazuno, Keiko, Shibasaki, Ken-ichiro, Yamazaki, Yoji, Kaneko, Kenji, Ueda, Nobuo, Ohguchi, Masahiro, Ishihara, Yuichi, Noguchi, Toshihide, Nishihara, Tatsuji
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.09.1999
• Subjects: 2-aminoethanesulphonic acid; Actinobacillus actinomycetemcomitans; Aggregatibacter actinomycetemcomitans; Alveolar Bone Loss - prevention & control; Animals; Bone Marrow Cells; Bone resorption; Bone Resorption - pathology; Bone Resorption - prevention & control; Calcitriol - pharmacology; Calcium Channel Agonists - pharmacology; Cell Differentiation - drug effects; Cell Division - drug effects; Cell Survival - drug effects; Cells, Cultured; Cricetinae; Culture Techniques; Dentistry; Dinoprostone - physiology; Female; Hematopoietic Stem Cells - cytology; Humans; Inflammation Mediators - physiology; Interleukin-1 - physiology; Lipopolysaccharides - pharmacology; Mesocricetus; Mice; Osteoblasts - drug effects; osteoclasts; Osteoclasts - cytology; Osteoclasts - drug effects; periodontitis; Periodontitis - complications; Skull; Space life sciences; Taurine - pharmacology; Taurine - therapeutic use; Bone resorption; PGE 2, prostaglandin E2; 2-aminoethanesulphonic acid; Actinobacillus actinomycetemcomitans; osteoclasts; periodontitis; α-MEM, α-minimal essential medium; IL-1α, interleukin-1α; FCS, fetal calf serum
• ISSN: 0003-9969; 1879-1506
• DOI: 10.1016/S0003-9969(99)00067-9

It is known that bone resorption is mediated by osteoclasts, and lipopolysaccharide (LPS) and inflammatory mediators such as interleukin-1 (IL-1) and prostaglandin E 2 (PGE 2) induce osteoclast differentiation from haemopoietic cells, 2-aminoethanesulphonic acid, which is known as taurine, is an important nutrient and is added to most synthetic human infant milk formulas. In this study, it was found that 2-aminoethanesulphonic acid inhibits the stimulation of bone resorption mediated by LPS of the periodontopathic microorganism Actinobacillus actinomycetemcomitans Y4 in organ cultures of newborn mouse calvaria. The effect of 2-aminoethanesulphonic acid on the development and survival of osteoclast-like multinucleated cells produced in a mouse bone-marrow culture system was also examined. 2-aminoethanesulphonic acid (100 μg/ml) suppressed the formation of these osteoclast-like cells in the presence of LPS of A. actinomycetemcomitans Y4, IL-1α or PGE 2 in mouse marrow cultures. On the other hand, 2-aminoethanesulphonic acid did not inhibit 1α, 25-dihydroxyvitamin D 3-mediated osteoclast differentiation. Although IL-1α elongated the survival of the osteoclast-like cells, 2-aminoethanesulphonic acid blocked the supportive effect of IL-1α on osteoclast survival. 2-aminoethanesulphonic acid showed no effect on the growth of mouse osteoblasts. Finally, it was found that 2-aminoethanesulphonic acid inhibited alveolar bone resorption in experimental periodontitis in hamsters. These results suggest that 2-aminoethanesulphonic acid is an effective agent in preventing inflammatory bone resorption in periodontal diseases.