The influence of various modified nucleotides placed as 3′-dangling end on thermal stability of RNA duplexes

• Published in: Biophysical chemistry Vol. 97; no. 2; pp. 243 - 249
• Main Authors: Ziomek, Krzysztof, Kierzek, Elżbieta, Biała, Ewa, Kierzek, Ryszard
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 19.06.2002
• Subjects: DNA - chemistry; DNA - genetics; Hot Temperature; Modified oligoribonucleotides; Modified uridine; Nucleic Acid Heteroduplexes - chemistry; Oligoribonucleotides - chemical synthesis; RNA, Double-Stranded - chemistry; RNA, Double-Stranded - genetics; Thermodynamics; Thermodynamics of RNA duplexes; Uridine - chemistry; Modified uridine; Modified oligoribonucleotides; Thermodynamics of RNA duplexes
• ISSN: 0301-4622; 1873-4200
• DOI: 10.1016/S0301-4622(02)00073-X

The ribonucleic acids (RNA) form highly folded structures, which behind the helical fragments contain several secondary and tertiary structural motives. All of them have an influence on thermodynamic stability of the RNA. The 5′- and 3′-dangling ends are one of those structural motives, which effect stability of the adjacent helixes. In this paper, we described the influence of 14 different modified nucleotides, placed as 3′-dangling ends, on thermal stability of the RNA duplexes. Collected data demonstrate that: (i) 5-substituents of the uridine have an impact on the 3′-dangling end effect and the largest changes were observed for 5-chloro, bromo and methyl substituents; (ii) position of the methyl group within the uracil residue affect the thermal stability of the duplex; (iii) increasing a size of the heterocycle base placed as the 3′-terminal unpaired nucleotide enhances stabilization of duplexes.