Application of high-performance thin layer chromatography mass spectrometry (HPTLC MS) in foodomics authenticity

• Published in: Talanta open Vol. 9; p. 100315
• Main Authors: Sharma, Neha, Anurag, Singh, Harjeet, Sharma, Alok
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.08.2024; Elsevier
• Subjects: Authenticity; Chemometric; Food adulterants; Food authentication; HPTLC-MS, Food omics research; Authenticity; Food adulterants; Chemometric; Food authentication; HPTLC-MS, Food omics research
• ISSN: 2666-8319; 2666-8319
• DOI: 10.1016/j.talo.2024.100315

•HPTLC-MS techniques have the potential to be used in real time and have gained popularity in recent years.•The current work highlights pertinent and significant information regarding the hyphenated HPTLC-MS technique's ability to detect both food components and their adulterants.•The present work reviews the use of analytical methods in qualitative and quantitative studies of food components and their adulterants.•Study of recent advancements coupling of analytical techniques that can improve detection efficiency of food.•Hyphenated HPTLC-MS technique found to be useful in food industry for adulteration detections. Chemometric analysis is also a powerful tool that can be used in the preliminary stages of analytical methods optimization, but is also efficient in data processing. These new approaches may be the key to the analysis of proteins, carbohydrate, sphignolipids, polyphenols in different food components. Although there are challenges in identifying and annotating those compounds due to the limited availability of analytical standards and structural diversity. Chromatograms are a useful tool for learning more about the chemical make-up of the food being tested. This information can occasionally be implicit or presented in a subtle way. Therefore, to extract it, chemometric tools and data mining techniques are needed. A chromatogram's fingerprint allows for the possibility of performing both identity and quality testing on food. This viewpoint aims to offer a current assessment of chromatographic fingerprinting technique in the area of food authentication. Additionally, this methodology's limitations, absence from official analytical methods, and future directions are discussed. Novel techniques have been employed in the past few decades, ranging from high-pressure thin liquid chromatography (HPTLC) to mass spectrometry (MS) and other spectroscopic methods. In the last decade research developments, and the application of analytical methods in qualitative and quantitative studies of food components and their adulterants is reviewed in the present work. High-pressure Thin liquid chromatography (HPTLC) is hyphenated with high resolution mass spectrometry is particularly addressed due to its applicability in the targeted/untargeted metabolomic analysis of all food components. In the current study, the HPTLC-MS technique was used to examine food components and food adulteration. The HPTLC-MS hyphenated techniques were found to be an extremely helpful method for the detection of food adulterants as well as the various food components, such as protein, carbohydrates, lipids, essential oils, and flavonoids, which were found to be effective as medicinally. Application of HPTLC-MS method for the routine analysis of food vegetable analysis. The thodology have advantages to avoids various chemical steps and this techniques provides a simpler and rapid method for routine analysis of different types of samples. [Display omitted]