Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity

A chemically-synthesized gene and natural complementary DNA coding for human lymphotoxin were isolated and engineered for expression in Escherichia coli. Purified recombinant lymphotoxin shows cytotoxic activity on murine and human tumour cell lines in vitro and causes necrosis of certain murine sar...

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Bibliographic Details
Published inNature (London) Vol. 312; no. 5996; p. 721
Main Authors Gray, P W, Aggarwal, B B, Benton, C V, Bringman, T S, Henzel, W J, Jarrett, J A, Leung, D W, Moffat, B, Ng, P, Svedersky, L P
Format Journal Article
LanguageEnglish
Published England 01.01.1984
Subjects
Amino Acid Sequence
Animals
Base Sequence
Cells, Cultured
Cloning, Molecular
DNA - genetics
Gene Expression Regulation
Humans
Lymphokines - genetics
Lymphotoxin-alpha - genetics
Lymphotoxin-alpha - therapeutic use
Mice
Sarcoma, Experimental - therapy
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Summary:A chemically-synthesized gene and natural complementary DNA coding for human lymphotoxin were isolated and engineered for expression in Escherichia coli. Purified recombinant lymphotoxin shows cytotoxic activity on murine and human tumour cell lines in vitro and causes necrosis of certain murine sarcomas in vivo.
ISSN:0028-0836
DOI:10.1038/312721a0