Determination of aldehydes in food by high-performance liquid chromatography with biosensor coupling and micromembrane suppressors

A high-performance liquid chromatography system for the determination of aldehydes in food was developed incorporating an Cation MicroMembrane Suppressor (CMMS) and enzyme reactors packed with VA-Epoxy on which aldehyde dehydrogenase from bakers yeast and NADH oxidase from Bacillus licheniformis wer...

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Bibliographic Details
Published inJournal of Chromatography A Vol. 880; no. 1; pp. 233 - 242
Main Authors Schultheiss, J., Jensen, D., Galensa, R.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 02.06.2000
Subjects
Aldehydes
Aldehydes - analysis
Biosensing Techniques
Calibration
Chromatography, High Pressure Liquid - methods
Food Analysis - instrumentation
Food Analysis - methods
Furaldehyde
Hydroxymethylfuraldehyde
Membranes, Artificial
Hydroxymethylfuraldehyde
Detection, LC
Furaldehyde
Food analysis
Biosensors
Beverages
Aldehydes
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Summary:A high-performance liquid chromatography system for the determination of aldehydes in food was developed incorporating an Cation MicroMembrane Suppressor (CMMS) and enzyme reactors packed with VA-Epoxy on which aldehyde dehydrogenase from bakers yeast and NADH oxidase from Bacillus licheniformis were immobilized. The method was based on the principle that the separation efficiency of HPLC is combined with the sensitivity of electrochemical detection and the specificity of enzymes. Main attention was directed to the determination of 5-hydroxymethyl-2-furaldehyde and 2-furaldehyde, the occurence of which is an indication of quality deterioration in several food products. The efficiency of the method has been shown by the analysis of honey, coffee and related beverages, refreshments, sherry, port, dry fruits and breakfast cereals.
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ISSN:0021-9673
DOI:10.1016/S0021-9673(99)01086-9