Single-stranded DNA condensed with poly- l-lysine results in nanometric particles that are significantly smaller, more stable in physiological ionic strength fluids and afford higher efficiency of gene delivery than their double-stranded counterparts

Nonviral gene transfer vectors have been actively studied in the past years in order to obtain structural entities with minimum size and defined shape. The final size of a gene transfer vector, which is compacted into unimolecular complexes, is directly proportional to the mass of the nucleic acid t...

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Published inBiochimica et biophysica acta Vol. 1572; no. 1; pp. 37 - 44
Main Authors Molas, M, Bartrons, R, Perales, J.C
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 15.08.2002
Subjects
DNA, Single-Stranded - chemistry
Flow Cytometry
Gene Expression
Gene therapy
Genetic Therapy
Genetic Vectors
Microscopy, Electron
Nonviral gene transfer
Nucleic Acid Denaturation
Osmolar Concentration
Particle Size
Polylysine - chemistry
Receptor mediated endocytosis
Single-stranded DNA
Solutions
Transfection
Nonviral gene transfer
Receptor mediated endocytosis
Single-stranded DNA
Gene therapy
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Summary:Nonviral gene transfer vectors have been actively studied in the past years in order to obtain structural entities with minimum size and defined shape. The final size of a gene transfer vector, which is compacted into unimolecular complexes, is directly proportional to the mass of the nucleic acid to be compacted. Thus, the purpose of this study was to assess the possibility of producing ssDNA vectors and their biophysical and biological characterization. We have obtained ssDNA/poly- l-lysine complexes that are significantly smaller than their double-stranded counterparts. We have also identified a lesser aggregative behavior of compacted single-stranded vs. double-stranded DNA vectors in the presence of physiological NaCl concentrations. Expression of compacted ssDNA is observed in hepatoma cell lines. Moreover, we have successfully delivered galactosylated ssDNA complexes into cells that express the asialoglycoprotein receptor via receptor-mediated endocytosis. The reduced size and biophysical behavior of ssDNA vectors may provide an advantage for transfection of eukaryotic cells.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/S0304-4165(02)00276-3