Proteomic analysis of rat soleus and tibialis anterior muscle following immobilization

A proteomic analysis was performed comparing normal slow twitch type fiber rat soleus muscle and normal fast twitch type fiber tibialis anterior muscle to immobilized soleus and tibialis anterior muscles at 0.5, 1, 2, 4, 6, 8 and 10 days post immobilization. Muscle mass measurements demonstrate mass...

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Published inJournal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 769; no. 2; pp. 323 - 332
Main Authors Isfort, Robert J, Wang, Feng, Greis, Kenneth D, Sun, Yiping, Keough, Thomas W, Bodine, Sue C, Anderson, N.Leigh
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 05.04.2002
Subjects
Amino Acid Sequence
Animals
Hindlimb Suspension
Mass Spectrometry
Molecular Sequence Data
Muscle Proteins - analysis
Muscle Proteins - chemistry
Muscle, Skeletal - chemistry
Proteins
Proteome
Rats
Space life sciences
Proteins
Muscles
Proteomic analysis
Non-programmatic
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Summary:A proteomic analysis was performed comparing normal slow twitch type fiber rat soleus muscle and normal fast twitch type fiber tibialis anterior muscle to immobilized soleus and tibialis anterior muscles at 0.5, 1, 2, 4, 6, 8 and 10 days post immobilization. Muscle mass measurements demonstrate mass changes throughout the period of immobilization. Proteomic analysis of normal and atrophied soleus muscle demonstrated statistically significant changes in the relative levels of 17 proteins. Proteomic analysis of normal and atrophied tibialis anterior muscle demonstrated statistically significant changes in the relative levels of 45 proteins. Protein identification using mass spectrometry was attempted for all differentially regulated proteins from both soleus and tibialis anterior muscles. Four differentially regulated soleus proteins and six differentially regulated tibialis anterior proteins were identified. The identified proteins can be grouped according to function as metabolic proteins, chaperone proteins, and contractile apparatus proteins. Together these data demonstrate that coordinated temporally regulated changes in the proteome occur during immobilization-induced atrophy in both slow twitch and fast twitch fiber type skeletal muscle.
Bibliography:ObjectType-Article-1
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ISSN:1570-0232
1873-376X
DOI:10.1016/S1570-0232(02)00021-1