Evidence for the generation of an electronic excitation state(s) in human polymorphonuclear leukocytes and its participation in bactericidal activity

Heat-killed opsonized Propionibacterium, shermanii, ( P., shermanii) and opsonized live Staphylococcus, aureus, ( S., aureus) are shown to elicit a chemiluminescence (CL) from human polymorphonuclear leukocytes (PMN) but not from lymphocytes upon phagocytosis. The CL is correlated with hexose monoph...

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Published inBiochemical and biophysical research communications Vol. 47; no. 4; pp. 679 - 684
Main Authors Allen, Robert C., Stjernholm, Rune L., Steele, Richard H.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 26.05.1972
Subjects
Blood
Glycolysis
Hexosephosphates - metabolism
Hot Temperature
Humans
Latex
Leukocytes - metabolism
Luminescent Measurements
Lymphocytes
Microspheres
Models, Biological
Opsonin Proteins
Oxygen
Pentosephosphates - metabolism
Phagocytosis
Propionibacterium
Staphylococcus
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Summary:Heat-killed opsonized Propionibacterium, shermanii, ( P., shermanii) and opsonized live Staphylococcus, aureus, ( S., aureus) are shown to elicit a chemiluminescence (CL) from human polymorphonuclear leukocytes (PMN) but not from lymphocytes upon phagocytosis. The CL is correlated with hexose monophosphate (HMP) shunt activity, number of PMN, and bacteria. CL was evoked from PMN by opsonized latex particles. No CL was obtained from PMN exposed to serum alone, nor unopsonized bacteria. It is proposed that the CL of PMN reflects the generation of singlet oxygen, 10 2, which acts per, se , as the bactericidal and/or CL species.
Bibliography:ObjectType-Article-1
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ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(72)90545-1