Somatic embryo proliferation, maturation and germination in Catharanthus roseus

In the present study an efficient somatic embryogenesis method has been developed in Catharanthus roseus. Friable embryogenic callus was induced from hypocotyl of in vitro germinated seeds on Murashige and Skoog basal nutrient media supplemented with various auxins particularly 2,4-D (1.0 mg l-1). H...

Full description

Saved in:
Bibliographic Details
Published inPlant cell, tissue and organ culture Vol. 84; no. 3; pp. 325 - 332
Main Authors Junaid, A, Mujib, A, Bhat, M.A, Sharma, M.P
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 01.03.2006
Subjects
auxins
benzyladenine
Biological and medical sciences
Biotechnology
callus
Catharanthus roseus
culture media
dose response
Eukaryotic cell cultures
explants
fructose
Fundamental and applied biological sciences. Psychology
gibberellic acid
hypocotyls
in vitro culture
in vitro regeneration
maltose
methodology
Methods. Procedures. Technologies
micropropagation
Miscellaneous
naphthaleneacetic acid
Plant cells and fungal cells
seed germination
somatic embryogenesis
somatic embryos
Apocynaceae
Ripening
carbon sources
Vegetals
Dicotyledones
Germination
somatic embryogenesis
Angiospermae
Spermatophyta
Catharanthus roseus
Somatic embryo
Online AccessGet full text

Cover

More Information
Summary:In the present study an efficient somatic embryogenesis method has been developed in Catharanthus roseus. Friable embryogenic callus was induced from hypocotyl of in vitro germinated seeds on Murashige and Skoog basal nutrient media supplemented with various auxins particularly 2,4-D (1.0 mg l-1). However, only NAA (1.0 mg l-1) produced somatic embryos in cultures. Embryo proliferation was even high on the same medium added with BAP. Cotyledonary somatic embryo germinated and converted into plantlets in BAP (0.5 mg l-1) added medium following a treatment with gibberellic acid (1.0 mg l-1) for maturation. Carbon sources and concentrations had a marked influence on maturation process. Plantlet conversion was better achieved when embryos were matured on 3% fructose or 3-6% maltose. The result discussed in this paper indicates that somatic embryos were produced in numbers and converted plantlets can be used as raw material, genetic modification to embryo precursor cell may improve alkaloid yield further.
Bibliography:http://dx.doi.org/10.1007/s11240-005-9041-7
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-005-9041-7