Isoprostane levels in lipids extracted from atherosclerotic arteries of nonhuman primates
Nonhuman primates used in these studies had been fed for 5 years diets enriched with cholesterol and one of three classes of fatty acids: saturated, monounsaturated, or polyunsaturated fatty acids. Atherosclerotic iliac artery lipid extracts were quantitatively analyzed for cholesterol, cholesteryl...
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Published in | Free radical biology & medicine Vol. 30; no. 12; pp. 1337 - 1346 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
15.06.2001
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Subjects | |
Online Access | Get full text |
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Summary: | Nonhuman primates used in these studies had been fed for 5 years diets enriched with cholesterol and one of three classes of fatty acids: saturated, monounsaturated, or polyunsaturated fatty acids. Atherosclerotic iliac artery lipid extracts were quantitatively analyzed for cholesterol, cholesteryl esters, fatty acid composition, and a marker of lipid oxidation, the F
2-isoprostanes. There was no significant difference in the mean accumulation of F
2-isoprostanes among the different diet groups. To account for the small, individual variation in the arachidonate concentration the F
2-isoprostane mass from each sample was normalized by dividing by arachidonate mass: F
2-isoprostane mass/(mass arachidonate). At lower levels of cholesterol accumulation, the F
2-isoprostane mass/(mass arachidonate) ratio was greater in lipids from POLY arteries compared to SAT arteries, but the reverse was true at high levels of cholesterol. F
2-isoprostane/(mass arachidonate) increased with mole fraction linoleate for the SAT group, but decreased for the POLY group. In summary, these studies demonstrated that there is no simple explanation of how F
2-isoprostane accumulation did not depend on the concentration of oxidizable lipids that promote free-radical lipid oxidation. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0891-5849 1873-4596 |
DOI: | 10.1016/S0891-5849(01)00527-5 |