Recent advances in fluorescence correlation spectroscopy

Fluorescence correlation spectroscopy is a method in which fluctuations in the fluorescence arising from a very small sample volume are correlated to obtain information about the processes giving rise to the fluctuations. Recent progress has been made in methodologies such as two-photon excitation,...

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Bibliographic Details
Published inCurrent Opinion in Structural Biology Vol. 12; no. 5; pp. 634 - 641
Main Authors Thompson, Nancy L, Lieto, Alena M, Allen, Noah W
Format Book Review Journal Article
LanguageEnglish
Published England Elsevier Ltd 01.10.2002
Subjects
Cell-surface receptors
cross-correlation
fluorescence intensity distribution analysis
GFP
Green Fluorescent Proteins
image correlation spectroscopy
intracellular dynamics
Luminescent Proteins - chemistry
phospholipid membranes
photon counting histogram
Photons
Proteins - chemistry
Sensitivity and Specificity
Spectrometry, Fluorescence - instrumentation
Spectrometry, Fluorescence - methods
Spectrometry, Fluorescence - trends
total internal reflection
two-photon excitation
Biotechnology
cross-correlation
image correlation spectroscopy
Biochemistry
phospholipid membranes
GFP
two-photon excitation
Techniques & Methods
photon counting histogram
fluorescence intensity distribution analysis
intracellular dynamics
Structural biology
Cell-surface receptors
total internal reflection
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Summary:Fluorescence correlation spectroscopy is a method in which fluctuations in the fluorescence arising from a very small sample volume are correlated to obtain information about the processes giving rise to the fluctuations. Recent progress has been made in methodologies such as two-photon excitation, photon counting histogram analysis, cross-correlation, image correlation and evanescent excitation. Fluorescence correlation spectroscopy techniques have been applied to several biological processes, including fluorescent protein photodynamics, binding equilibria and kinetics, protein oligomerization, nucleic acid interactions, and membrane and intracellular dynamics. Various applications and development studies indicate the likelihood that fluorescence correlation spectroscopy will prove to be broadly applicable not only for characterizing processes in solution but also for examining processes in membranes and intact cells
Bibliography:ObjectType-Article-1
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ObjectType-Review-2
ISSN:0959-440X
1879-033X
DOI:10.1016/S0959-440X(02)00368-8