Induction of CMV-1 promoter by 4-hydroxy-2-nonenal in human embryonic kidney cells

Oxidative stress, i.e., excessive production of oxygen free radicals and reactive oxygen species, leads to lipid peroxidation and to formation of reactive aldehydes which act as second messengers of free radicals. It has previously been shown that oxidative stress may be involved in the transcriptio...

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Published inActa biochimica Polonica Vol. 57; no. 2; pp. 179 - 183
Main Authors Jaganjac, Morana, Matijevic, Tanja, Cindric, Marina, Cipak, Ana, Mrakovcic, Lidija, Gubisch, Wolfgang, Zarkovic, Neven
Format Journal Article
LanguageEnglish
Published Poland 01.01.2010
Subjects
Acrolein
Aldehydes
Aldehydes - pharmacology
Cell Line
Cell Survival
Cysteine Proteinase Inhibitors - pharmacology
Cytomegalovirus
Cytomegalovirus - drug effects
Cytomegalovirus - genetics
Free radicals
Gene regulation
Humans
Hydrogen peroxide
Kidney - cytology
Lipid peroxidation
Oxidative stress
oxygen free radicals
Plasmids
Promoter Regions, Genetic
Promoters
Reactive oxygen species
Second messengers
Transcription
Virus Activation - drug effects
yellow fluorescent protein
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Summary:Oxidative stress, i.e., excessive production of oxygen free radicals and reactive oxygen species, leads to lipid peroxidation and to formation of reactive aldehydes which act as second messengers of free radicals. It has previously been shown that oxidative stress may be involved in the transcriptional regulation of cytomegalovirus (CMV) immediate early promoter, involved in viral reactivation from latency. In the current study we used a plasmid containing the yellow fluorescent protein (YFP) gene under the control of CMV-1 promoter to monitor the influence of hydrogen peroxide and reactive aldehydes, 4-hydroxy-2-nonenal (HNE) and acrolein, on CMV-1 promoter activation in human embryonic kidney cells (HEK293). While acrolein was ineffective, hydrogen peroxide slightly (50 %) stimulated the CMV promoter. In contrast, HNE had a strong, up to 3-fold, enhancing effect on the CMV-1 promoter within four as well as after 24h of treatment. The most effective was the treatment with 24 microM HNE. This effect of HNE suggests that stressful conditions associated with lipid peroxidation could lead to CMV activation.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0001-527X
1734-154X
DOI:10.18388/abp.2010_2392