Isolation and analysis of candidate ascochyta blight defence genes in chickpea. Part II. Microarray expression analysis of putative defence-related ESTs

• Published in: Physiological and molecular plant pathology Vol. 66; no. 5; pp. 201 - 210
• Main Authors: Coram, Tristan E., Pang, Edwin C.K.
• Format: Journal Article
• Language: English
• Published: London Elsevier India Pvt Ltd 01.05.2005; Elsevier
• Subjects: Agronomy. Soil science and plant productions; Ascochyta; Ascochyta blight; Ascochyta rabiei; Biological and medical sciences; Chickpea; Cicer arietinum; Classical and quantitative genetics. Population genetics. Molecular genetics; EST; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Generalities. Genetics. Plant material; Genes. Genome; Genetics and breeding of economic plants; Genomics; Microarray; Molecular and cellular biology; Molecular genetics; Pathology, epidemiology, host-fungus relationships. Damages, economic importance; Pest resistance; Phytopathology. Animal pests. Plant and forest protection; Plant pathogens; Varietal selection. Specialized plant breeding, plant breeding aims; Chickpea; Ascochyta blight; Microarray; Genomics; EST; Plant pathology; Plant pathogen; DNA chip; Molecular marker; Genetic determinism; Fungi; Cicer arietinum; Dicotyledones; Angiospermae; Candidate gene; Fungi Imperfecti; Ascochyta rabiei; Thallophyta; Pathophysiology; Gene expression; Defense; Fungus resistance; Grain legume; Leguminosae; Vegetable crop; Analysis; Isolation; Defense mechanism; Spermatophyta; Molecular biology; Expressed sequence tag
• ISSN: 0885-5765; 1096-1178
• DOI: 10.1016/j.pmpp.2005.08.002

Using microarray technology and previously identified defence-related ESTs from chickpea ( Cicer arietinum L.), the ascochyta blight ( Ascochyta rabiei (Pass.) Labrousse) resistance response was studied in a highly resistant chickpea accession (ICC3996) and a susceptible cultivar (Lasseter). The time-series expression patterns of 20 defence-related ESTs were studied after inoculation with A. rabiei. Ten of the defence-related ESTs displayed up- or down-regulation in ICC3996 and/or Lasseter compared to uninoculated control samples. Hierarchical clustering grouped the ESTs into clusters of similar observations, revealing that three defence related ESTs showed differential up-regulation in ICC3996 when compared to Lasseter—a leucine zipper protein, SNAKIN2 antimicrobial peptide precursor, and elicitor-induced receptor protein. The potential involvement of these ESTs in effective chickpea defence against ascochyta blight is discussed. The microarray expression analysis of defence-related ESTs in chickpea represents the first study of this type, and future work will focus on large-scale expression studies aimed at further elucidating the function of genes involved in ascochyta blight resistance and the pathway of their action.