Callus cultures of Genista plants—in vitro material producing high amounts of isoflavones of phytoestrogenic activity
Callus cultures of six Genista species were established with the objective to produce isoflavones of phytoestrogenic activity. The cultures were optimised for their growth and isoflavonoid production by changing various media in the presence or absence of light. The best growth and the highest isofl...
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Published in | Plant science (Limerick) Vol. 165; no. 5; pp. 1101 - 1108 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Shannon
Elsevier Ireland Ltd
01.11.2003
Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | Callus cultures of six
Genista species were established with the objective to produce isoflavones of phytoestrogenic activity. The cultures were optimised for their growth and isoflavonoid production by changing various media in the presence or absence of light. The best growth and the highest isoflavone production was obtained under constant light regime on SH basal medium containing 22.6 μmol l
−1 2,4-dichlorophenoxyacetic acid (2,4-D), 23.2 μmol l
−1 kinetin and 3% w/v of sucrose. Callus cultures of all species produced more isoflavones than the parent herbs. The isoflavonoid group produced was composed of 14 compounds with a clear domination of genistin. In vitro cultures had lower contents of genistein esters than the herbs—possible causes are discussed. The callus with the highest isoflavone content was obtained from
Genista tinctoria, producing 6586.5 mg of total isoflavones per 100 g DW, in which HPLC analysis identified 3016.3 mg of genistin. |
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ISSN: | 0168-9452 1873-2259 |
DOI: | 10.1016/S0168-9452(03)00305-4 |