Flow cytometric determination of radiation-induced chromosome damage and its correlation with cell survival

Chinese hamster M3-1 cells were irradiated with several doses of X rays or alpha particles from 238Pu. Propidium iodide-stained chromosome suspensions were prepared at different times after irradiation; cells were also assayed for survival. The DNA histograms of these chromosomes showed increased ba...

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Bibliographic Details
Published inRadiation research Vol. 99; no. 1; p. 44
Main Authors Welleweerd, J, Wilder, M E, Carpenter, S G, Raju, M R
Format Journal Article
LanguageEnglish
Published United States 01.07.1984
Subjects
Alpha Particles
Animals
Cell Cycle
Cell Line
Cell Survival - radiation effects
Chromosome Aberrations
Cricetinae
Cricetulus
Dose-Response Relationship, Radiation
Energy Transfer
Flow Cytometry
Plutonium
Radiation Genetics
Time Factors
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Summary:Chinese hamster M3-1 cells were irradiated with several doses of X rays or alpha particles from 238Pu. Propidium iodide-stained chromosome suspensions were prepared at different times after irradiation; cells were also assayed for survival. The DNA histograms of these chromosomes showed increased background counts with increased doses of radiation. This increase in background was cell-cycle dependent and was correlated with cell survival. The correlation between radiation-induced chromosome damage and cell survival was the same for X rays and alpha particles. Data are presented which indicate that flow cytometric analysis of chromosomes of irradiated cell populations can be a useful adjunct to classical cytogenic analysis of irradiation-induced chromosomal damage by virtue of its ability to express and measure chromosomal damage not seen by classical cytogenic methods.
ISSN:0033-7587
DOI:10.2307/3576445