Comparison of serum iohexol clearance and plasma creatinine clearance in clinically normal horses

• Published in: American journal of veterinary research Vol. 70; no. 12; pp. 1545 - 1550
• Main Authors: Wilson, Katherine E, Wilcke, Jeff R, Crisman, Mark V, Ward, Daniel L, McKenzie, Harold C, Scarratt, W. Kent
• Format: Journal Article
• Language: English
• Published: United States 01.12.2009
• Subjects: Animals; Area Under Curve; blood plasma; blood sampling; blood serum; contrast agents; Contrast Media - pharmacokinetics; creatinine; Creatinine - blood; Creatinine - metabolism; drug evaluation; glomerular filtration rate; Hemibody Irradiation; horses; Horses - blood; intravenous injection; iohexol; Iohexol - pharmacokinetics; Kidney - metabolism; kidneys; time series analysis
• ISSN: 0002-9645; 1943-5681
• DOI: 10.2460/ajvr.70.12.1545

Objective-To determine whether a limited sampling time method based on serum iohexol clearance (Cl(iohexol)) would yield estimates of glomerular filtration rate (GFR) in clinically normal horses similar to those for plasma creatinine clearance (Cl(creatinine)). Animals-10 clinically normal adult horses. Procedures-A bolus of iohexol (150 mg/kg) was administered IV, and serum samples were obtained 5, 20, 40, 60, 120, 240, and 360 minutes after injection. Urinary clearance of exogenous creatinine was measured during three 20-minute periods. The GFR determined by use of serum Cl(iohexol) and plasma Cl(creatinine) was compared with limits of agreement plots. Results-Values obtained for plasma Cl(creatinine) ranged from 1.68 to 2.69 mL/min/kg (mean, 2.11 mL/min/kg). Mean serum Cl(iohexol) was 2.38 mL/min/kg (range, 1.95 to 3.33 mL/min/kg). Limits of agreement plots indicated good agreement between the methods. Conclusions and Clinical Relevance-Use of serum Cl(iohexol) yielded estimates of GFR in clinically normal adult horses similar to those for plasma Cl(creatinine). This study was the first step in the evaluation of the use of serum Cl(iohexol) for estimating GFR in adult horses.