In planta analysis of the Agrobacterium tumefaciens T-cyt gene promoter: identification of an upstream region essential for promoter activity in leaf, stem and root cells of transgenic tobacco

• Published in: Plant molecular biology Vol. 22; no. 5; p. 923
• Main Authors: Neuteboom, S.T.C. (Leiden Univ. (Netherlands). Vakgroep Moleculaire Plantenwetenschappen), Hulleman, E, Schilperoort, R.A, Hoge, J.H.C
• Format: Journal Article
• Language: English
• Published: Netherlands 01.08.1993
• Subjects: AGROBACTERIUM TUMEFACIENS; Agrobacterium tumefaciens - genetics; Cloning, Molecular; Cytokinins - genetics; DNA, Bacterial; FEUILLE; FUSION DE GENES; GENE; GENE FUSION; GENES; HOJAS; LEAVES; NICOTIANA; Nicotiana - genetics; Nicotiana - microbiology; NUCLEOTIDE SEQUENCE; PLANTAS TRANSGENICAS; PLANTE TRANSGENIQUE; Plants, Genetically Modified; Plants, Toxic; Promoter Regions, Genetic; Protoplasts; RACINE; RAICES; Regulatory Sequences, Nucleic Acid; ROOTS; SECUENCIA NUCLEICA; Sequence Deletion; SEQUENCE NUCLEIQUE; STEMS; TALLO; TIGE; Transfection; TRANSGENIC PLANTS
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/BF00027379

The promoter region of the Agrobacterium tumefaciens T-cyt gene was fused to a beta-glucuronidase (gusA) reporter gene and introduced into tobacco plants. Detection of gusA expression in transgenic F1 progeny revealed that the T-cyt promoter is active in many, if not all, cell types in leaves, stems and roots of fully developed plants. Developmental stage-dependent promoter activity was observed in seedlings. Analysis of 5'-deleted promoter fragments showed that sequences located between positions -185 and -139 with respect to the T-cyt translational start codon are essential for T-cyt promoter activity in transfected tobacco protoplasts as well as in transformed tobacco plants.