High-resolution sub-cellular imaging by correlative NanoSIMS and electron microscopy of amiodarone internalisation by lung macrophages as evidence for drug-induced phospholipidosis

Correlative NanoSIMS and EM imaging of amiodarone-treated macrophages shows the internalisation of the drug at a sub-cellular level and reveals its accumulation within the lysosomes, providing direct evidence for amiodarone-induced phospholipidosis. Chemical fixation using tannic acid effectively se...

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Published inChemical communications (Cambridge, England) Vol. 53; no. 9; pp. 1506 - 1509
Main Authors Jiang, Haibo, Passarelli, Melissa K, Munro, Peter M G, Kilburn, Matt R, West, Andrew, Dollery, Colin T, Gilmore, Ian S, Rakowska, Paulina D
Format Journal Article
LanguageEnglish
Published England 2017
Subjects
Amiodarone - chemistry
Amiodarone - pharmacology
Anti-Arrhythmia Agents - chemistry
Anti-Arrhythmia Agents - pharmacology
Correlation
Drugs
Humans
Image resolution
Imaging
Lung - cytology
Lung - drug effects
Lysosomes
Lysosomes - chemistry
Lysosomes - metabolism
Macrophages
Macrophages - drug effects
Microscopy, Electron
Nanostructure
Nanotechnology
Phospholipids - metabolism
Spectrometry, Mass, Secondary Ion
Tannic acid
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Summary:Correlative NanoSIMS and EM imaging of amiodarone-treated macrophages shows the internalisation of the drug at a sub-cellular level and reveals its accumulation within the lysosomes, providing direct evidence for amiodarone-induced phospholipidosis. Chemical fixation using tannic acid effectively seals cellular membranes aiding intracellular retention of diffusible drugs.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1359-7345
1364-548X
DOI:10.1039/c6cc08549k