Description of Proteiniphilum saccharofermentans sp. nov., Petrimonas mucosa sp. nov. and Fermentimonas caenicola gen. nov., sp. nov., isolated from mesophilic laboratory-scale biogas reactors, and emended description of the genus Proteiniphilum

• Published in: International journal of systematic and evolutionary microbiology Vol. 66; no. 3; pp. 1466 - 1475
• Main Authors: Hahnke, Sarah, Langer, Thomas, Koeck, Daniela E, Klocke, Michael
• Format: Journal Article
• Language: English
• Published: England 01.03.2016
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.000902

Three novel, facultatively anaerobic bacteria of the family (phylum ) were isolated from mesophilic laboratory-scale biogas reactors. The strains were Gram-negative rods. Optimal growth occurred between 35 and 45 °C and at pH 7.1-7.8. The main fermentation products were acetic and propionic acids. The predominant fatty acid in all strains was anteiso-C , and the only respiratory quinone detected was menaquinone MK-8. 16S rRNA gene sequence comparison indicated that strains M3/6 and ING2-E5B were most closely related to the type strain of , with sequence similarities of 97.3 and 94.5 %. Strain ING2-E5A showed the closest affiliation to the type strain of , with 97 % sequence identity. DNA-DNA hybridization of strain M3/6 and ING2-E5A with the most closely related type strains showed 43.3-45.6 and 23.8-25.7 % relatedness, respectively, which supports the conclusion that both isolates represent novel species. Phylogenetic analysis and comparison of cellular fatty acid patterns indicated that strain ING2-E5B cannot be classified as a member of any previously described genus. Therefore, because of the physiological, genotypic and chemotaxonomic differences, it is proposed to designate novel species within the genera and , sp. nov. (type strain M3/6  = DSM 28694  = CECT 8610  = LMG 28299 ) and sp. nov. (type strain ING2-E5A  = DSM 28695  = CECT 8611 ), and a novel species of a new genus, gen. nov., sp. nov. (type strain of is ING2-E5B  = DSM 28696  = CECT 8609  = LMG 28429 ). In addition, an emended description of the genus is provided.