Cloning of rat telomerase catalytic subunit functional domains, reconstitution of telomerase activity and enzymatic profile of pig and chicken tissues

• Published in: Life sciences (1973) Vol. 73; no. 21; pp. 2749 - 2760
• Main Authors: Wong, Stephen C.H, Ong, Lee Lee, Er, Connie P.N, Gao, Shujun, Yu, Hanry, So, Jimmy B.Y
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 10.10.2003
• Subjects: Animals; Catalytic Domain - genetics; Cells, Cultured; Chick Embryo; Cloning, Molecular; DNA-Binding Proteins; Embryo, Mammalian - cytology; Embryo, Mammalian - enzymology; Fibroblasts - cytology; Fibroblasts - enzymology; Gene Expression Regulation, Developmental; Humans; Mice; Rat telomerase catalytic subunit (rTERT); Rats; RNA - metabolism; RT–PCR; Species Specificity; Swine; Telomerase - genetics; Telomerase - metabolism; Telomerase activity; RT–PCR; Rat telomerase catalytic subunit (rTERT); Telomerase activity
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/S0024-3205(03)00670-2

Telomerase is a ribonucleoprotein polymerase which adds TTAGGG repeats to telomeric ends. Recent studies reported the reverse transcription enzyme activity mostly from the catalytic subunit (TERT) of the enzyme complex. Both human telomerase catalytic subunit (hTERT) and mouse telomerase catalytic subunit (mTERT) had been previously cloned but not rat telomerase catalytic subunit rTERT. In this study, the rTERT functional domains were cloned and was found that its function resemble to mouse and human telomerase. In addition, chicken and pig telomerase activity profile were studied and its enzyme activity is related to its proliferation capability of individual tissues. However, its catalytic subunit does not like mouse, rat and human cases that the telomerase activity could not reconstituted by the in-vitro transfection of mTERT and hTERT cloned vectors. Here we demonstrated that rTERT is similar to mTERT and hTERT but not pig and chicken telomerase. Further studies are needed to verify the malignancy characteristics because nowadays artificial organs/tissues from these animals are used for the transplantation to human body.