Apoptosis and Expression of AQP5 and TGF-β in the Irradiated Rat Submandibular Gland

• Published in: Cancer research and treatment Vol. 41; no. 3; pp. 145 - 154
• Main Authors: Choi, Jin Hwa, Wu, Hong-Gyun, Jung, Kyeong Cheon, Lee, Seung Hee, Kwon, Eun Kyung
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Cancer Association 01.09.2009; 대한암학회
• Subjects: Original; 의약학; Submandibular gland; TGF-β; AQP5; Radiation; Apoptosis
• ISSN: 1598-2998; 2005-9256
• DOI: 10.4143/crt.2009.41.3.145

To evaluate the effect of X-ray irradiation on apoptosis and change of expression of aquaporin 5 (AQP5) and transforming growth factor-β(TGF-β) in the rat submandibular gland (SMG). SMGs of 120 male Sprague-Dawley rats were irradiated with a single X-ray dose (3, 10, 20, or 30 Gy). At the early and late post-irradiation phase, apoptosis was measured by the terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) method, and expression of AQP5 and TGF-β was determined by immunohistochemical staining. At the late post-irradiation phase, increased apoptosis was evident and marked decreases of expression of AQP5 expression by acinar cells and TGF-β expression by ductal cells were evident. Apoptosis after X-ray irradiation develops relatively late in rat SMG. Irradiation reduces AQP5 and TGF-β expression in different SMG cell types.