Detection of CD4＋ T-lymphocytes from hemodialyzed patients by surface plasmon resonance

• Published in: Chinese chemical letters Vol. 23; no. 12; pp. 1393 - 1395
• Main Authors: Wang, Hai Yan, Li, Jian Jun, Cao, Xiao Na, Xu, Ji Ying, Liu, Mei Rong, Chen, Yi
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2012; School of Chemistry and Chemical Engineering, Shanxi Datong University, Datong 037009, China%East Section of Dongzhimen Hospital Affiliated, Beijing 101100, China%Community Health Service of Beijing Sport University, Beijing 100190, China%Key Laboratory of Analytical Chemistry for Living Biosystems, ICCAS, Beijing 100190, China
• Subjects: Antibody–antigen interactions; CD4; CD4+ T-lymphocytes; Hemodialyzed patients; SPR; T-淋巴细胞; 患者; 检测; 淋巴细胞亚群; 蛋白质分泌; 血液透析; 表面等离子体共振; Hemodialyzed patients; SPR; CD4+ T-lymphocytes; Antibody–antigen interactions; Antibody-antigen interactions
• ISSN: 1001-8417; 1878-5964
• DOI: 10.1016/j.cclet.2012.10.017

A surface plasmon resonance （SPR） method was presented to discriminate hemodialyzed T-lymphocytes from the normal based on antibody--cell recognition. By dynamic reaction with fixed anti-human CD4 antibody, SPR could offer significant signals to distinguish hemodialyzed patients from the healthy controls within 200 s after the cell injection in respect of either rising speed or maximum binding capacity （p 〈 0.01）. The ratio method is also used to exclude the non-specific adsorption. The percentage of hemodialyzed patients＇ CD4＋ T ceils against the healthy control is 69 ± 18%. The most attractive of the present method is its ability to detect the intact and label-free lymphocytes, and further to detect the subpopulations, or proteins secreted by the desired lymphocytes subset.