Chiral separation of thiazide diuretics by HPLC on Chiralcel OD-RH®, Chiralcel OJ-R® and Chirobiotic-T™® phases

• Published in: Journal of biochemical and biophysical methods Vol. 53; no. 1; pp. 15 - 24
• Main Authors: Visegrády, Balázs, Konecsni, Tünde, Grobuschek, Nina, Schmid, Martin G, Kilár, Ferenc, Aboul-Enein, Hassan Y, Gübitz, Gerald
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2002
• Subjects: Benzoates; Benzothiadiazines; Carbamates; Cellulose - analogs & derivatives; Cellulose-based stationary phase; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Diuretics; Enantiomer separation; Equipment Design; Phenylcarbamates; Reproducibility of Results; Sensitivity and Specificity; Sodium Chloride Symporter Inhibitors - chemistry; Sodium Chloride Symporter Inhibitors - isolation & purification; Stereoisomerism; Teicoplanin; Teicoplanin chiral stationary phase; Cellulose-based stationary phase; Diuretics; Teicoplanin chiral stationary phase; Enantiomer separation
• ISSN: 0165-022X; 1872-857X
• DOI: 10.1016/S0165-022X(02)00088-X

This contribution deals with comparative studies on the chiral separation of thiazide diuretics using cellulose tris(3,5-dimethylphenylcarbamate) (Chiralcel OD-RH®), cellulose tris(4-methylbenzoate) (Chiralcel OJ-R®) and teicoplanin (Chirobiotic T™®) phases. All columns showed good chiral recognition ability for this class of compounds. Out of seven compounds investigated, six were resolved with baseline resolution with at least one of the three columns.