Neuronal contact guidance in magnetically aligned fibrin gels: effect of variation in gel mechano-structural properties

• Published in: Biomaterials Vol. 22; no. 10; pp. 1065 - 1075
• Main Authors: Dubey, N., Letourneau, P.C., Tranquillo, R.T.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.05.2001
• Subjects: Aligned fibrin; Animals; Axon; Biocompatible Materials; Biomechanical Phenomena; Cell Adhesion; Chick Embryo; Contact guidance; Fibrin; Fibrin gel; Ganglia, Spinal - cytology; Gels; Magnetic alignment; Magnetics; Materials Testing; Microscopy, Confocal; Nerve regeneration; Nerve Regeneration - physiology; Neurite; Neurites - ultrastructure; Neurons - cytology; Neurite; Aligned fibrin; Magnetic alignment; Contact guidance; Axon; Fibrin gel; Nerve regeneration
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/S0142-9612(00)00341-0

Neurite outgrowth from chick dorsal root ganglia entrapped in isotropic and magnetically aligned fibrin gels was studied, and the dependence on the diameter of the fibrin fibrils was characterized. The fibrin fibril diameter was varied, as inferred from turbidity measurements, by using different Ca 2+ concentrations in the fibrin-forming solution, but this variation was accomplished without affecting the degree of magnetic-induced alignment, as directly visualized in fluorescently spiked gels. Magnetically aligned fibrin gels possessing different fibril diameters but similar alignment resulted in drastic changes in the contact guidance response of neurites, with no response in gels formed in 1.2 m m Ca 2+ (having smaller fibril diameter, ca. 150 nm), but a strong response in gels formed in 12 and 30 m m Ca 2+ (having larger fibril diameter, ca. 510 nm) with an attendant two-fold increase in neurite length. These changes are attributed to variation of the mechano-structural properties of the network of aligned fibrils as the fibril diameter is varied.