Serum levels of tetranectin, intercellular adhesion molecule-1 and interleukin-10 in B-chronic lymphocytic leukemia

• Published in: Clinical biochemistry Vol. 32; no. 8; pp. 639 - 645
• Main Authors: Kamper, Elli F, PapahilisS, Anastasios D, Angelopoulou, Maria K, Kopeikina, Ludmila T, Siakantaris, Marina P, Pangalis, Gerassimos A, Stavrudis, John C
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.1999
• Subjects: Aged; Aged, 80 and over; B-CLL; Biomarkers, Tumor - blood; Blood Proteins - analysis; cICAM1; Enzyme-Linked Immunosorbent Assay; Female; Humans; IL-10; Intercellular Adhesion Molecule-1 - blood; Interleukin-10 - blood; Lectins, C-Type; Leukemia, Lymphocytic, Chronic, B-Cell - blood; Leukemia, Lymphocytic, Chronic, B-Cell - classification; Leukemia, Lymphocytic, Chronic, B-Cell - diagnosis; Leukemia, Lymphocytic, Chronic, B-Cell - pathology; Male; Middle Aged; Neoplasm Staging; Reagent Kits, Diagnostic; Reference Values; Sensitivity and Specificity; tetranectin; cICAM1; tetranectin; IL-10; B-CLL
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/S0009-9120(99)00066-1

Background and objective: The fibrinolytic regulator tetranectin (TN), in association with the circulating intercellular adhesive molecule-1 (cICAM-1) and interleukin -10 (IL-10), may be involved in the metastatic cascade of B-chronic lymphocytic leukemia (B-CLL). Our aim was to investigate the potential usefulness of these molecules as prognostic markers in B-CLL. Design and methods: Therefore, TN, cICAM-1, and IL-10 were assessed (ELISA) in the serum of 53 B-CLL patients, classified in Binet A, B, and C stages in comparison with those in 45 healthy subjects (HS). Results: TN was significantly lower in B-CLL patients than in HS (9.63 [8.75–11.51] mg/L, 13.75 [12.56–14.64] ng/mL, respectively, p < 10 −5), being lower ( p = 0.05) in B and C stage patients (subgroup B+C) than in A stage ones (subgroup A). cICAM-1 levels were significantly higher in B-CLL patients than in HS (475.86 [355.86–593.79] ng/mL vs. 225.62 [118.49–312.83] ng/mL, respectively, p < 10 −5) with a tendency for higher levels in subgroup B+C than in subgroup A. A significant correlation of cICAM-1 with lactate dehydrogenase (LDH) ( r s = 0.532, p = 0.049), and a significant increase in cICAM-1 in B-CLL with diffuse bone marrow infiltration (BMI) compared to that in B-CLL with nondiffuse BMI (624.48 [557.24–726.55] ng/mL vs. 480.34 [368.96–590.34] ng/mL, respectively, p = 0.0172) were found. A significant negative correlation between TN and cICAM-1 ( r s = −0.5017, p = 0.0001) was observed. IL-10 was detected in all B-CLL patients and in no HS (7.37 [5.30–10.55] pg/mL), being higher ( p = 0.0153) in C than in A stage patients. A significant correlation of IL-10 with TN and cICAM-1 in subgroup B+C ( r s = −0.659 [ p = 0.014] and r s = 0.679 [ p = 0.011], respectively) was found. Conclusions: The abovementioned findings and good performance characteristics of TN and cICAM-1 in B-CLL suggest the potential usefulness of these adhesive/recognition molecules as prognostic markers in B-CLL. The implication of these molecules along with IL-10 in the disease process deserves further study.