Enhanced 5-lipoxygenase activity in lung macrophages compared to monocytes from normal subjects

• Published in: The Journal of immunology (1950) Vol. 138; no. 5; pp. 1546 - 1550
• Main Authors: Bigby, TD, Holtzman, MJ
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 01.03.1987; American Association of Immunologists
• Subjects: Arachidonate 5-Lipoxygenase - metabolism; Arachidonate Lipoxygenases - metabolism; Arachidonic Acid; Arachidonic Acids - metabolism; Biological and medical sciences; Calcimycin - pharmacology; Cell Differentiation; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Hydroxyeicosatetraenoic Acids - metabolism; Immunobiology; Leukotriene B4 - biosynthesis; Lung - cytology; Macrophages - cytology; Macrophages - enzymology; Monocytes - cytology; Monocytes - enzymology; Myeloid cells: ontogeny, maturation, markers, receptors; Human; Enzymatic activity; Monocyte; Arachidonic acid derivatives; Lung; Chemical mediator; Inflammation; Lipoxygenase; Comparative study; Blood; Macrophage; Phagocyte
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.138.5.1546

We compared lipoxygenase activities of lung macrophages obtained from bronchoalveolar lavage to activities of blood monocytes purified by using discontinuous plasma/Percoll density gradients and adherence to tissue culture plastic in five normal subjects. Cells were incubated with ionophore A23187 (10(-9) to 10(-5) M) or arachidonic acid (0.12 to 80 microM) for 1 to 60 min at 37 degrees C to construct dose-response and time-dependence curves of lipoxygenase product generation. Products were identified and were quantified by using high-pressure liquid chromatography and ultraviolet spectroscopy. Under all conditions of product generation, both macrophages and monocytes generated predominantly (5S,12R)-dihydroxy-(6Z, 8E, 10E, 14Z)-eicosatetraenoic acid (leukotriene B4 (LTB4] and (5S)-hydroxy-(6E, 8Z, 11Z, 14Z) - eicosatetraenoic acid (5 - HETE), but, in each subject, macrophages invariably released greater amounts of LTB4 and 5-HETE than monocytes. In response to A23187, macrophages released a maximum of 183 +/- 96 pmol of LTB4 and 168 +/- 108 pmol of 5-HETE per 10(6) cells (mean +/- SEM), whereas monocytes released only 16 +/- 1 and 18 +/- 8 pmol per 10(6) cells of LTB4 and 5-HETE, respectively. After adding arachidonic acid, macrophages released a maximum of 52 +/- 21 pmol of LTB4 and 223 +/- 66 pmol of 5-HETE, whereas monocytes released no detectable products. The results suggest that mononuclear phagocyte maturation in the lung may be accompanied by an enhanced ability to generate 5-lipoxygenase products.