Association between DNA strand breakage and oxidative, inflammatory and endothelial biomarkers in type 2 diabetes

• Published in: Mutation research Vol. 732; no. 1-2; p. 16
• Main Authors: Tatsch, Etiane, Bochi, Guilherme V, Piva, Sílvia J, De Carvalho, José A M, Kober, Helena, Torbitz, Vanessa D, Duarte, Thiago, Signor, Cristiane, Coelho, Adriane C, Duarte, Marta M M F, Montagner, Greice F F S, Da Cruz, Ivana B M, Moresco, Rafael N
• Format: Journal Article
• Language: English
• Published: Netherlands 01.04.2012
• Subjects: Biomarkers - blood; Case-Control Studies; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - genetics; Diabetes Mellitus, Type 2 - pathology; DNA Breaks, Double-Stranded; Endothelial Cells - metabolism; Endothelium, Vascular - pathology; Female; Humans; Inflammation Mediators - blood; Male; Middle Aged; Oxidation-Reduction; Oxidative Stress
• ISSN: 1873-135X
• DOI: 10.1016/j.mrfmmm.2012.01.004

Evidence has been presented recently that type 2 diabetes patients have an increased level of DNA damage. This DNA damage could be associated with oxidative, inflammatory, and endothelial biomarkers and could represent a possible indication of injury in the endothelium and induction of inflammation in type 2 diabetes. To confirm this possible association, DNA strand breakage was evaluated by use of the comet assay and its association with oxidative, inflammatory, and endothelial biomarkers in type 2 diabetes patients. A case-control study (30 healthy controls and 32 subjects with type 2 diabetes) was performed to evaluate the association between DNA damage and NOx (nitrate/nitrite), interleukin-6 (IL-6), urinary albumin, fasting glucose, and glycated hemoglobin (HbA(1c)) levels. Type 2 diabetes patients presented higher DNA damage than control subjects, higher levels of IL-6 and urinary albumin, and lower NOx. Significant correlations between DNA damage and NOx (r=-0.303, p=0.016), IL-6 (r=0.845, p<0.001), urinary albumin (r=0.496, p<0.001), fasting glucose (r=0.449, p<0.001), and HbA(1c) (r=0.575, p<0.001) were reported. Our findings showed an increase of DNA damage in type 2 diabetes especially in those patients with poor glycemic control and associations among NOx, IL-6 and urinary albumin levels with DNA damage.