Hydrogen peroxide regulation of bovine endothelin-converting enzyme-1

• Published in: Free radical biology & medicine Vol. 32; no. 5; pp. 406 - 413
• Main Authors: López-Ongil, S, Saura, M, Zaragoza, C, Gónzalez-Santiago, L, Rodrı́guez-Puyol, M, Lowenstein, C.J, Rodrı́guez-Puyol, D
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2002
• Subjects: Animals; Antioxidants - pharmacology; Aorta - metabolism; Aspartic Acid Endopeptidases - genetics; Aspartic Acid Endopeptidases - metabolism; Blotting, Western; Catalase - metabolism; Cattle; Cell Nucleus; Cells, Cultured; Cytosol; DNA Primers - chemistry; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Electrophoretic Mobility Shift Assay; Endothelin converting enzyme; Endothelin-1 - metabolism; Endothelin-Converting Enzymes; Endothelium, Vascular - cytology; Endothelium, Vascular - enzymology; Endothelium, Vascular - metabolism; Free radical; Gene Expression Regulation, Enzymologic - physiology; Glucose Oxidase - pharmacology; HeLa Cells; Humans; Hydrogen peroxide; Hydrogen Peroxide - pharmacology; Luciferases - metabolism; Metalloendopeptidases; Polymerase Chain Reaction; Promoter Regions, Genetic - genetics; Reactive oxygen species; Reactive Oxygen Species - metabolism; RNA, Messenger - analysis; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Sequence Deletion; STAT 3 transcription factor; STAT3 Transcription Factor; Trans-Activators - genetics; Trans-Activators - metabolism; Transfection; ECE-1; H 2O 2; Reactive oxygen species; STAT; Hydrogen peroxide; BAEC; Endothelin converting enzyme; STAT 3 transcription factor; ROS; GO; Free radical
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/S0891-5849(01)00822-X

Vascular injury leads to the production of reactive oxygen species (ROS), but the mechanisms by which ROS contribute to vascular pathology are not completely understood. We hypothesized that ROS increase endothelin converting enzyme (ECE-1) expression. We found that glucose oxidase (GO) increases ECE-1 mRNA, protein, and activity in bovine aortic endothelial cells. Catalase abolishes this effect. Glucose oxidase treatment of endothelial cells transactivates the ECE-1 promoter. The ECE-1 promoter element that mediates this response to GO is located between −444 and −216 bp. This region contains a STAT response element, and GO activates STAT-3 binding to this STAT response element. Our data suggest that STAT3 mediates hydrogen peroxide induction of ECE-1 expression.