Synthesis and crystal structure of two new dinuclear cobalt(II) complexes interaction with HeLa cells

• Published in: European journal of medicinal chemistry Vol. 46; no. 1; pp. 160 - 167
• Main Authors: Gao, En-jun, Gao, Xia-nan, Guan, Feng, Zhu, Ming-chang, Liu, Lei, Zhang, Min, Zhang, Ying-xing, Wang, Ying, Wen, Zheng, Zhang, Yan, Zhang, Ying, Liang, Qi
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.01.2011
• Subjects: Antineoplastic Agents - chemical synthesis; Antineoplastic Agents - chemistry; Antineoplastic Agents - metabolism; Antineoplastic Agents - pharmacology; Apoptosis - drug effects; Apoptotic; Carboxylic Acids - chemistry; Cell Survival - drug effects; Cleavage; Co(II) coordinate complexes; Cobalt - chemistry; Crystallography, X-Ray; Cytotoxicity in vitro; DNA - isolation & purification; DNA - metabolism; Extracted DNA binding; HeLa Cells; Humans; Ligands; Models, Molecular; Molecular Conformation; Organometallic Compounds - chemical synthesis; Organometallic Compounds - chemistry; Organometallic Compounds - metabolism; Organometallic Compounds - pharmacology; Spectrometry, Fluorescence; Cleavage; Cytotoxicity in vitro; Extracted DNA binding; Apoptotic; Co(II) coordinate complexes
• ISSN: 0223-5234; 1768-3254
• DOI: 10.1016/j.ejmech.2010.10.031

Two novel dinuclear complexes [Co2(L)2(phen)2·2H2O]n (1), [Co2(L)2(bipy)2]n (2) (where phen=1,10-phenanthroline, bipy=2,2′-bipyridine, and L=biphenyl-2,4′-dicarboxylic acid) have been synthesized and characterized using IR, 1H NMR, element analysis and single-crystal X-ray diffractometry. The binding of the complexes with HC-DNA (HeLa cells DNA, which was extracted by ourselves) was investigated by fluorescence spectrum. The experimental results show that the two complexes have the ability to bind with extracted HC-DNA. Gel electrophoresis assay demonstrates the ability of the complexes to cleave the extracted HC-DNA. The complexes exhibit a higher cytotoxicity against tumor cells as against normal cells in vitro. Further more, the apoptotic tests indicate that the complexes have an apoptotic effect on HeLa cells. Two Co(II) atoms’ coordinate pattern is the same, both Co(II) atoms are six coordinated by two nitrogen atoms from the bipy, four oxygen atoms from four different biphenyl-2,4′-dicarboxylic acid ligands. [Display omitted] ►We synthesis two novel dinuclear Co(II) complexes, and extracted HC-DNA (HeLa cells DNA). ►The binding behaviors of the complexes with extracted HC-DNA were investigated by fluorescence spectroscopy. ►Their cleavage behavior toward extracted HC-DNA, cytotoxicity in vitro and apoptosis were also investigated.