The use of fluorescent phorbol esters in studies of protein kinase C–membrane interactions

• Published in: Chemistry and physics of lipids Vol. 116; no. 1; pp. 75 - 91
• Main Authors: Slater, Simon J, Ho, Cojen, Stubbs, Christopher D
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.06.2002
• Subjects: Animals; Ca 2+-binding; Cell Membrane - metabolism; Fluorescent Dyes; Fluorescent phorbol esters; Humans; Liposomes - metabolism; Phorbol Esters - chemistry; Phospholipids - metabolism; Protein Binding; Protein Kinase C - chemistry; Protein Kinase C - metabolism; Protein kinase C–membrane interaction; OAG, 1-oleoyl-2-acetyl- sn-glycerol; BPS, bovine brain phosphatidylserine; Dansyl-PS, N-(5-dimethylaminonaphthalene-1-sulfonyl)-dioleoyl-phosphatidylserine; Protein kinase C–membrane interaction; Bryo-1, bryostatin-1; Dansyl-TPA, 4β-12- O-(12-dansylaminododecanoyl) phorbol-13-acetate; SAPD, sapintoxin D; PDBu, phorbol-12, 13-dibutyrate; PLPC, 1-palmitoyl-2-linoleoyl- sn-glycero-3-phosphocholine; nPKCε, ‘novel’ protein kinase C; LUV, large unilamellar vesicles; POPE, 1-palmitoyl-2-oleoyl- sn-glycero-3-phosphoethanolamine; aPKC, ‘atypical’ protein kinase C; PE, phosphatidylethanolamine; MBP 4–14, myelin basic protein peptide substrate; DAG, 1,2-dioleoyl- sn-glycerol; 4α-TPA, 4α-12- O-tetradecanoylphorbol-13-acetate; POPS, 1-palmitoyl-2-oleoyl- sn-glycero-3-phosphoserine; POPC, 1-palmitoyl-2-oleoyl- sn-glycero-3-phosphocholine; TPA, 4β-12- O-tetradecanoylphorbol-13-acetate; Ca 2+-binding; NBD-PS, 1-palmitoyl-2-[ N-(7-nitro-2-1, 3-benzoxadiazole-4-yl)]- sn-glycero-3-phosphoserine; Fluorescent phorbol esters; ε-Peptide, peptide substrate based on the cPKCε pseudosubstrate; cPKC, ‘conventional’ protein kinase C
• ISSN: 0009-3084; 1873-2941
• DOI: 10.1016/S0009-3084(02)00021-X

The family of protein kinase C (PKC) isozymes belongs to a growing class of proteins that become active by associating with membranes containing anionic phospholipids, such as phosphatidylserine. Depending on the particular PKC isoform, this process is mediated by Ca 2+-binding to a C2 domain and interaction of activators such as 1,2-diacyl-sn-glycerol or phorbol esters with tandem C1 domains. This cooperation between the C1 and C2 domains in inducing the association of PKC with lipid membranes provides the energy for a conformational change that consists of the release of a pseudosubstrate sequence from the active site, culminating in activation. Thus, the properties of the interactions of the C1 and C2 domains with membranes, both as isolated domains, and as modules in the full length PKC isoforms, have been the subject of intense scrutiny. Here, we review the findings of studies in which fluorescent phorbol esters have been utilized to probe the properties of the C1 domains of PKC with respect to the interaction with activators, the subsequent interaction with membranes, and the role of the activating conformational change that leads to activation.