Wickerhamiella nakhonpathomensis f.a. sp. nov., a novel ascomycetous yeast species isolated from a mushroom and a flower in Thailand

• Published in: International journal of systematic and evolutionary microbiology Vol. 72; no. 1
• Main Authors: Khunnamwong, Pannida, Kingphadung, Kanokwan, Lomthong, Thanasak, Kanpiengjai, Apinun, Khanongnuch, Chartchai, Limtong, Savitree
• Format: Journal Article
• Language: English
• Published: England 01.01.2022
• Subjects: Agaricales; DNA, Fungal - genetics; DNA, Ribosomal Spacer - genetics; Fatty Acids - chemistry; Flowers - microbiology; Mycological Typing Techniques; Phylogeny; Saccharomycetales - classification; Saccharomycetales - isolation & purification; Sequence Analysis, DNA; Thailand; Thailand; Wickerhamiella nakhonpathomensis f.a. sp. nov; Thailand; mushroom; yeast; inflorescence
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.005191

Strains SU22 (TBRC 14875 ) and FLA11.5, representing a novel anamorphic yeast species, were respectively isolated from a fruiting body of a species and an inflorescence of a species collected in Thailand. Analysis of the sequences of the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) regions showed that the two strains differed by two nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and were identical in the ITS regions. CBS 8459 was the most closely related species, but with 24-26 nucleotide substitutions in the D1/D2 domains of the LSU rRNA gene and 24 nucleotide substitutions in the ITS regions. A phylogenetic analysis, based on the sequences of the D1/D2 domains, indicated that the two strains represented a species in the genus which was distinct from other recognized species of the genus. Therefore, the two strains were assigned as a novel species, for which we propose the name f.a. sp. nov. The holotype is TBRC 14875 (isotype PYCC 8914 ). The MycoBank number of the novel species is MB 840833.