Oxidative modification of serum albumin in an experimental glycation model of diabetes mellitus in vitro: Effect of the pyridoindole antioxidant stobadine

Under conditions of an experimental in vitro glycation model, the pyridoindole antioxidant stobadine significantly inhibited glycation-related fluorescence changes of bovine serum albumin as well as the yield of 2,4-dinitrophenyl-hydrazine-reactive carbonyls with an efficacy comparable to that of th...

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Bibliographic Details
Published inLife sciences (1973) Vol. 65; no. 18; pp. 1995 - 1997
Main Authors Stefek, M., Krizanova, L., Trnkova, Z.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Inc 01.10.1999
Subjects
Animals
antioxidant
Antioxidants - chemistry
Carbolines - chemistry
diabetic complications
Fluorescence
Glycosylation
Oxidation-Reduction
protein glyco-oxidation
Serum Albumin, Bovine - chemistry
stobadine
diabetic complications
antioxidant
protein glyco-oxidation
stobadine
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Summary:Under conditions of an experimental in vitro glycation model, the pyridoindole antioxidant stobadine significantly inhibited glycation-related fluorescence changes of bovine serum albumin as well as the yield of 2,4-dinitrophenyl-hydrazine-reactive carbonyls with an efficacy comparable to that of the reference antioxidants Trolox C and 2-keto-4-methiolbutyric acid, and more efficiently than did aminoguanidine. Since stobadine did not affect the covalent binding of glucose, the protective effect may be explained by the ability of the drug to eliminate free radical intermediates of glyco-oxidation reactions, operative after the preceding glycation steps.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0024-3205
1879-0631
DOI:10.1016/S0024-3205(99)00463-4