Molecular genetic relationships between Bombycidae and Saturniidae based on the mitochondria DNA encoding of large and small rRNA

• Published in: Genetic analysis : biomolecular engineering Vol. 15; no. 6; pp. 223 - 228
• Main Authors: Hwang, Jae-Sam, Lee, Jin-Sung, Goo, Tae-Won, Young Yun, Eun, Sohn, Hae-Ryong, Rak Kim, Ho, Kwon, O-Yu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.12.1999
• Subjects: Animals; Antheraea pernyi; Antheraea yamamai; Base Sequence; Bombyx - classification; Bombyx - genetics; Bombyx mandarina; Bombyx mori; DNA, Mitochondrial - genetics; DNA, Ribosomal - genetics; Molecular Sequence Data; Moths - classification; Moths - genetics; Phylogenetic relationships; Phylogeny; Polymerase Chain Reaction; RNA, Ribosomal - genetics; RNA, Ribosomal, 16S - genetics; rRNA 12S; rRNA 16S; rRNA gene; Saturniidae; Sequence Alignment; Bombyx mandarina; Phylogenetic relationships; Bombyx mori; Antheraea pernyi; Antheraea yamamai; rRNA gene
• ISSN: 1050-3862
• DOI: 10.1016/S1050-3862(99)00008-X

The phylogenetic relationships between Bombycidae ( Bombyx mori and Bombyx mandarina) and Saturniidae ( Antheraea yamamai and Antheraea pernyi) were investigated based on large and small mitochondiral rRNA genes. About 430 bp of four kinds of PCR-amplified fragments were sequenced and aligned. For the 16S rRNA gene, B. mori shared a 98, 87 and 86% sequence homology with B. mandarina, A. yamamai and A. pernyi, and for the 12S rRNA gene, B. mori shared a 99, 89 and 88% sequence homology with B. mandarina, A. yamamai and A. pernyi, respectively. DNA sequence data were also used for a phylogenetic analysis. All of the trees showed monophyly for both Bombycidae and Saturniidae. The monophyly confidence limits of these trees were estimated using bootstrapping tests and measured more than 99% for all trees for both Bombycidae and Saturniidae.