Production of fertile transgenic maize by electroporation of suspension culture cells

Fertile, transgenic maize plants were generated by electroporation of suspension culture cells that were treated with a pectin-degrading enzyme. Electroporation of cells from two different suspension cultures, one derived from A188 X B73 and one derived from a B73-related inbred, with a plasmid cont...

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Published inPlant molecular biology Vol. 24; no. 1; p. 51
Main Authors Laursen, C.M. (Plant Genetics DEKALB, Mystic, CT (USA)), Krzyzek, R.A, Flick, C.E, Anderson, P.C, Spencer, T.M
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.1994
Subjects
Blotting, Southern
CAL
CALLO
CALLUS
Cells, Cultured
Electroporation
FERTILIDAD
FERTILITE
FERTILITY
GENE
GENES
Genes, Plant
GENETIC TRANSFORMATION
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants, Genetically Modified
Reproduction
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
Transformation, Genetic
TRANSGENIC PLANTS
ZEA MAYS
Zea mays - cytology
Zea mays - genetics
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Summary:Fertile, transgenic maize plants were generated by electroporation of suspension culture cells that were treated with a pectin-degrading enzyme. Electroporation of cells from two different suspension cultures, one derived from A188 X B73 and one derived from a B73-related inbred, with a plasmid containing the bar gene, resulted in high-frequency recovery of stably transformed callus lines. Plants were regenerated from thirteen transformed callus lines and transmission of bar to progeny was demonstrated.
Bibliography:F30
9402298
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00040573