New approaches in the development of DNA sensors: hybridization and electrochemical detection of DNA and RNA at two different surfaces

• Published in: Bioelectrochemistry (Amsterdam, Netherlands) Vol. 56; no. 1; pp. 85 - 90
• Main Authors: Paleček, E, Fojta, M, Jelen, F
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.05.2002
• Subjects: Biosensing Techniques; DNA - chemistry; DNA sensors; Electrochemical detection; Electrochemistry - methods; Hybridization; Magnetic beads; Nucleic Acid Hybridization; RNA - chemistry; Sensitivity and Specificity; Magnetic beads; Electrochemical detection; Hybridization; DNA sensors
• ISSN: 1567-5394
• DOI: 10.1016/S1567-5394(02)00025-7

Up to now, the development of the electrochemical DNA hybridization sensors relied on solid electrodes, on which both the hybridization and detection steps have been performed. Here we propose a new method in which the DNA hybridization is performed at commercially available magnetic beads and electrochemical detection on detection electrodes (DE). Due to minimum nonspecific DNA adsorption at the magnetic beads, very high specificity of the DNA hybridization is achieved. Optimum DE can be chosen only with respect to the given electrode process. It is shown that high sensitivity and specificity in the detection of relatively long target DNAs can be obtained (a) by using cathodic stripping voltammetry at mercury or solid mercury amalgam DEs for the determination of purine bases, released from DNA by acid treatment, and (b) by enzyme-linked immunoassay of target DNA modified by osmium tetroxide,2,2′-bipyridine (Os,bipy) at carbon DEs. Direct determination of Os,bipy at mercury and carbon electrodes is also possible.