Sydowia polyspora associated with current season needle necrosis (CSNN) on true fir ( Abies spp.)

• Published in: Fungal biology Vol. 114; no. 7; pp. 545 - 554
• Main Authors: Talgø, Venche, Chastagner, Gary, Thomsen, Iben Margrete, Cech, Thomas, Riley, Kathy, Lange, Kurt, Klemsdal, Sonja Sletner, Stensvand, Arne
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.07.2010
• Subjects: Abies - microbiology; Abies grandis; Abies nordmanniana; Abies procera; Ascomycota - genetics; Ascomycota - isolation & purification; Ascomycota - physiology; Christmas trees; Hormonema dematioides; Kabatina abietis; Molecular Sequence Data; Plant Diseases - microbiology; Plant Leaves - microbiology; Seasons; Trees - microbiology; Hormonema dematioides; Abies grandis; Abies procera; Christmas trees; Abies nordmanniana; Kabatina abietis
• ISSN: 1878-6146; 1878-6162
• DOI: 10.1016/j.funbio.2010.04.005

Current season needle necrosis (CSNN) has been a serious foliage disorder on true fir Christmas trees and bough material in Europe and North America for more than 25 y. Approximately 2–4 weeks after bud break, needles develop chlorotic spots or bands that later turn necrotic. The symptoms have been observed on noble fir ( Abies procera), Nordmann fir ( A. nordmanniana) and grand fir ( A. grandis) on both continents. CSNN was reported as a physiological disorder with unknown aetiology from USA, Denmark, and Ireland, but was associated with the fungus Kabatina abietis in Germany, Austria and Norway. In 2007, a fungus that morphologically resembled K. abietis was isolated from symptomatic needle samples from Nordmann fir from Austria, Denmark, Germany, Norway, and USA. Sequencing of the internal transcribed spacer (ITS) region of ribosomal DNA of these cultures, plus a K. abietis reference culture from Germany (CBS 248.93), resulted in Hormonema dematioides, the imperfect stage of Sydowia polyspora, and thus the taxonomy is further discussed. Inoculation tests on Nordmann fir seedlings and transplants with isolates of S. polyspora from all five countries resulted in the development of CSNN symptoms. In 2009, S. polyspora was also isolated from symptomatic needles from Nordmann fir collected in Slovakia.