The presence of d-β-aspartic acid-containing peptides in elastic fibers of sun-damaged skin: a potent marker for ultraviolet-induced skin aging

Biologically uncommon d-aspartyl residues have been reported in proteins of various elderly tissues. We prepared a polyclonal antibody against d-β-Asp-containing peptide and examined its immunoreactivity in the skin. The antibody recognized integrated or disintegrated elastic fibers in the sun-expos...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 294; no. 5; pp. 1047 - 1051
Main Authors Fujii, Noriko, Tajima, Shingo, Tanaka, Nobuhiko, Fujimoto, Norihiro, Takata, Takumi, Shimo-Oka, Tadashi
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 28.06.2002
Subjects
Aged
Aging
Aging - radiation effects
Aspartic Acid - analysis
Biomarkers - analysis
Child
d-Aspartic acid
D-Aspartic Acid - analysis
D-Aspartic Acid - immunology
Elastic fiber
Elastic Tissue - chemistry
Face - anatomy & histology
Face - radiation effects
Humans
Immunohistochemistry
Isoaspartic Acid - analysis
Isoaspartic Acid - immunology
Middle Aged
Peptides - chemistry
Proteins - chemistry
Skin
Skin - anatomy & histology
Skin - radiation effects
Sun damage
Ultraviolet Rays - adverse effects
β-aspartic acid
Immunohistochemistry
d-Aspartic acid
β-aspartic acid
Aging
Elastic fiber
Skin
Sun damage
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Summary:Biologically uncommon d-aspartyl residues have been reported in proteins of various elderly tissues. We prepared a polyclonal antibody against d-β-Asp-containing peptide and examined its immunoreactivity in the skin. The antibody recognized integrated or disintegrated elastic fibers in the sun-exposed skin but not in the sun-protected skin of the elderly donors. Western blot analysis of the proteins isolated from sun-damaged skin demonstrated that the 50 kDa protein was immunoreactive with both antibodies for d-β-Asp-containing peptide and elastin. Ultraviolet (UV) irradiation on normal skin caused the appearance of d-β-Asp-containing peptide-immunoreactive fibers in the dermis. These results suggest that UV irradiation is closely related to the formation of d-β-Asp in the elastic fibers of skin. We propose that the antibody could be a useful indicator for sun damage of the skin.
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ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(02)00597-1