Proteomic analysis of endogenous conjugated linoleic acid biosynthesis in lactating rats and mouse mammary gland epithelia cells (HC11)

• Published in: Biochimica et biophysica acta Vol. 1804; no. 4; pp. 745 - 751
• Main Authors: Jin, Yong-Cheng, Lee, Hong-Gu, Xu, Cheng-Xiong, Han, Jeng-A, Choi, Seong-Ho, Song, Man-Kang, Kim, Young-Jun, Lee, Ki-Beom, Kim, Seon-Ku, Kang, Han-Seok, Cho, Byung-Wook, Shin, Teak-Soon, Choi, Yun-Jaie
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2010
• Subjects: Amino Acid Sequence; Animals; Cell Line; Conjugated linoleic acid; Electrophoresis, Gel, Two-Dimensional; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Female; Lactation - metabolism; Linoleic Acids, Conjugated - biosynthesis; Mammary Glands, Animal - cytology; Mammary Glands, Animal - drug effects; Mammary Glands, Animal - metabolism; Metabolic Networks and Pathways; Mice; Models, Biological; Molecular Sequence Data; Oleic Acids - pharmacology; Peroxiredoxins - genetics; Peroxiredoxins - metabolism; Polymerase Chain Reaction; Protein Disulfide-Isomerases - genetics; Protein Disulfide-Isomerases - metabolism; Proteomics; Rats; Rats, Sprague-Dawley; Real-time PCR; Receptors, Laminin - genetics; Receptors, Laminin - metabolism; Ribosomal Proteins - genetics; Ribosomal Proteins - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Spectrometry, Mass, Electrospray Ionization; Stearoyl-CoA desaturase; Tandem Mass Spectrometry; Trans vaccenic acid; Two-dimensional electrophoresis; Conjugated linoleic acid; LPL; Stearoyl-CoA desaturase; Two-dimensional electrophoresis; PRDX2; PDI; Real-time PCR; SCD; Trans vaccenic acid; CLA; FAS; LAMR1; TVA
• ISSN: 1570-9639; 0006-3002; 1878-1454
• DOI: 10.1016/j.bbapap.2009.11.016

This study was conducted to investigate the amount of CLA synthesized endogenously by rat mammary tissues in response to TVA (a precursor for cis-9, trans-11 CLA endogenous synthesis) treatment as well as the differences in the protein expression of genes encoding the biosynthesis of CLA in rat mammary tissue and mouse mammary gland epithelia cells (HC11). Treatment with TVA resulted in improved CLA productivity. Furthermore, 2-DE revealed two spots in samples of mammary tissues and one spot in samples of mammary gland epithelia cells (HC11) that were consistently altered in the TVA treatment groups when compared with the control group (non-fatty acid). The mRNA expression patterns of three of the proteins (PDI, PRDX2, LAMR1), as measured by real-time PCR, were similar to the pattern of protein abundance. In addition, the expression of SCD mRNA in the mammary tissue of rats and HC11 cell treated with TVA was higher than in the control group. Our results suggest that the identified proteins may be related to CLA biosynthesis in mammary tissue.