Growth Inhibition of Human Glioma Cells by Superinduced Human Interferon-β
Superinduction of human interferon-β(HuIFN-β) from human glioma cells has greater cytotoxicity than purified HuIFN-β derived from fibroblasts. However, superinduction requires several reagents like polyI:polyC, cycloheximide, and actinomycin D, which may contaminate the conditioned medium and obscur...
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Published in | Neurologia medico-chirurgica Vol. 35; no. 10; pp. 719 - 722 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Japan
The Japan Neurosurgical Society
1995
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Subjects | |
Online Access | Get full text |
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Summary: | Superinduction of human interferon-β(HuIFN-β) from human glioma cells has greater cytotoxicity than purified HuIFN-β derived from fibroblasts. However, superinduction requires several reagents like polyI:polyC, cycloheximide, and actinomycin D, which may contaminate the conditioned medium and obscure the effect of superinduced HuIFN-β. The present study used minimum doses of polyI:polyC and cycloheximide without actinomycin D to superinduce HuIFN-β. The superinduced HuIFN-β was purified by passing the medium through molecular sieve column chromatography. Fractionation of the eluate provided semipurified superinduced HuIFN-β which demonstrated a growth inhibitory effect against both the U251-MG autologous human glioma cell line and the SK-MG-1 homologous glioma cell line. This effect was neutralized by addition of anti-HuIFN-β monoclonal antibody (YSB-1). In a separate experiment, combinations of superinduction reagents were found not to have growth inhibitory effects because all inhibition in superinduced medium was completely neutralized by YSB-1. Superinduced HuIFN-β has a pure growth inhibitory effect on both autologous and homologous glioma cells, so may affect autocrine secretion of cytokines. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0470-8105 1349-8029 |
DOI: | 10.2176/nmc.35.719 |