Adsorptive Depletion of CD14+CD16+ Proinflammatory Monocyte Phenotype in Patients With Generalized Pustular Psoriasis: Clinical Efficacy and Effects on Cytokines

Generalized pustular psoriasis (GPP) is a subtype of psoriasis with strong association with activated neutrophils. Adsorptive granulocyte and monocyte apheresis (GMA) is an extracorporeal intervention for selective depletion of activated granulocytes and monocytes. However, the immunological mechani...

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Published inTherapeutic apheresis and dialysis Vol. 16; no. 5; pp. 436 - 444
Main Authors Fujisawa, Tomomi, Murase, Kana, Kanoh, Hiroyuki, Takemura, Masao, Ohnishi, Hidenori, Seishima, Mariko
Format Journal Article
LanguageEnglish
Published Melbourne, Australia Blackwell Publishing Asia 01.10.2012
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Summary:Generalized pustular psoriasis (GPP) is a subtype of psoriasis with strong association with activated neutrophils. Adsorptive granulocyte and monocyte apheresis (GMA) is an extracorporeal intervention for selective depletion of activated granulocytes and monocytes. However, the immunological mechanism(s) for the effect of GMA on patients is not fully defined yet. We investigated the effects of GMA on the ratio of CD14+CD16+ proinflammatory monocytes/CD14+ monocytes and cytokine/chemokine production by these leukocytes including CXCL8, CCL2, CCL3, CCL4, CCL5 and tumor necrosis factor (TNF)‐α in five patients with active GPP. CD14+CD16+ monocytes were significantly elevated in patients with active GPP, and GMA markedly reduced the CD14+CD16+/CD14+ ratio together with improvement of patients' clinical symptoms. The serum levels of CXCL8, CCL3 and CCL4 were increased in active GPP patients. Likewise, CCL2 production from monocytes was increased in active GPP patients. Further, CCL3 and CCL4 production from monocytes in active GPP patients were reduced after a course of GMA. Serum CCL5 level and the release of CCL5 from monocytes in active GPP were significantly reduced, but TNF‐α level in active GPP was similar to controls. Based on these results, we believe that in addition to neutrophils, elevated CD14+CD16+ proinflammatory monocytes are part of the immune pathology in GPP. Accordingly, selective depletion of CD14+CD16+ monocytes by GMA should be therapeutic in this condition.
Bibliography:istex:2E77D16011BE9FDE5A2F07C41989EF1BDD152AC2
ark:/67375/WNG-QM5L0W1G-2
ArticleID:TAP1108
Presented in part at the 32nd Annual Meeting of the Japanese Society for Apheresis held 30 October to 2 November 2011 in Tokyo, Japan.
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:1744-9979
1744-9987
DOI:10.1111/j.1744-9987.2012.01108.x