DNA-Dependent RNA-Directed Protein Synthesis in vitro, IV. Peptide Analysis of an in vitro and in vivo φ X174 Structural Protein
An antibody-precipitable structural protein from the bacteriophage φ X174 was purified from an in vitro protein-synthesizing system primed with the replicative DNA of the virus. This protein was trypsin digested in the presence of the differentially labeled analogous protein that was purified from t...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 67; no. 1; pp. 13 - 17 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences of the United States of America
01.09.1970
National Acad Sciences |
Subjects | |
Online Access | Get full text |
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Summary: | An antibody-precipitable structural protein from the bacteriophage φ X174 was purified from an in vitro protein-synthesizing system primed with the replicative DNA of the virus. This protein was trypsin digested in the presence of the differentially labeled analogous protein that was purified from the bacteriophage. The tryptic hydrolysate was subjected to high resolution peptide chromatography which revealed essentially identical patterns for the φ X174 proteins synthesized in vitro and in vivo. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Requests for reprints may be addressed to Dr. D. H. Gelfand, Department of Biology, University of California, San Diego, Revelle College, La Jolla, Calif. 92037. This work was supported, in part, by a U.S. Public Health Service research grant (GM-12934) and a National Science Foundation research grant (GB-11873). One of us (D.H.G.) was the recipient of a U.S. Public Health Service predoctoral traineeship grant (GM-00702) and the other (M.H.) is the recipient of a career development research award from the U.S. Public Health Service (GM-42360). |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.67.1.13 |