Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD

Brown adipocytes dissipate energy through non-shivering thermogenesis mediated by UCP1 protein, hence representing a powerful target to overcome obesity due to energy surplus. However, brown adipocytes are scarce in adult humans, especially in obese subjects, urging the development of novel strategi...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1889; p. 25
Main Authors Chen, Jingjuan, Wang, Chao, Kuang, Shihuan
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
Adipocytes - cytology
Adipocytes - metabolism
Adipogenesis - genetics
Animals
Cell Line
Cell Transdifferentiation - genetics
CRISPR-Cas Systems
Fluorescent Antibody Technique
Gene Editing
Gene Expression
Gene Targeting
Humans
Immunohistochemistry
Mice, Knockout
MyoD Protein - genetics
MyoD Protein - metabolism
RNA, Guide, CRISPR-Cas Systems
Satellite Cells, Skeletal Muscle - cytology
Satellite Cells, Skeletal Muscle - metabolism
Myoblast
Adipocyte
CRISPR/Cas9
Satellite cell
Transdifferentiation
Adipogenesis
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Summary:Brown adipocytes dissipate energy through non-shivering thermogenesis mediated by UCP1 protein, hence representing a powerful target to overcome obesity due to energy surplus. However, brown adipocytes are scarce in adult humans, especially in obese subjects, urging the development of novel strategies to boost the number of these thermogenic adipocytes from a therapeutical perspective. In this regard, transdifferentiation of myoblasts into brown adipocytes represents a promising approach. Here, we describe a method that we have recently developed to transdifferentiate myoblasts into brown adipocytes through CRISPR/Cas9-medidated targeting of MyoD, the master myogenic regulatory factor.
ISSN:1940-6029
DOI:10.1007/978-1-4939-8897-6_3