Dendritic cells treated with chloroquine modulate experimental autoimmune encephalomyelitis
Chloroquine (CQ), an antimalarial drug, has been shown to modulate the immune system and reduce the severity of experimental autoimmune encephalomyelitis (EAE). The mechanisms of disease suppression are dependent on regulatory T cell induction, although Tregs‐independent mechanisms exist. We aimed t...
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Published in | Immunology and cell biology Vol. 92; no. 2; pp. 124 - 132 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Nature Publishing Group
01.02.2014
Blackwell Science Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | Chloroquine (CQ), an antimalarial drug, has been shown to modulate the immune system and reduce the severity of experimental autoimmune encephalomyelitis (EAE). The mechanisms of disease suppression are dependent on regulatory T cell induction, although Tregs‐independent mechanisms exist. We aimed to evaluate whether CQ is capable to modulate bone marrow‐derived dendritic cells (DCs) both phenotypically and functionally as well as whether transfer of CQ‐modulated DCs reduces EAE course. Our results show that CQ‐treated DCs presented altered ultrastructure morphology and lower expression of molecules involved in antigen presentation. Consequently, T cell proliferation was diminished in coculture experiments. When transferred into EAE mice, DC‐CQ was able to reduce the clinical manifestation of the disease through the modulation of the immune response against neuroantigens. The data presented herein indicate that chloroquine‐mediated modulation of the immune system is achieved by a direct effect on DCs and that DC‐CQ adoptive transfer may be a promising approach for avoiding drug toxicity. |
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Bibliography: | These authors contributed equally to this work. |
ISSN: | 0818-9641 1440-1711 |
DOI: | 10.1038/icb.2013.73 |