In vivo cytotoxicity analysis of bioceramic root canal sealers on zebrafish embryo

• Published in: Journal of dental research, dental clinics, dental prospects Vol. 17; no. 4; pp. 242 - 249
• Main Authors: Ja'apar, Siti Aisyah Nadirah, Ichwan, Solachuddin Jauhari Arief, Mustaffa, Musliana
• Format: Journal Article
• Language: English
• Published: Iran Tabriz University of Medical Sciences 01.01.2023
• Subjects: Animal models; bioceramic root canal sealers; Calcium hydroxide; Calcium oxide; Calcium phosphates; Cell culture; Cell growth; Cell proliferation; Connective tissues; Cytotoxicity; Eggs; Embryos; Endodontics; Fourier transforms; in vivo; Infiltration; Inflammation; Mortality; Original; Root canals; Salicylic acid; Sealing compounds; Silicones; Solubility; Spectrum analysis; Toxicity; Zebrafish; zebrafish embryos; Zirconium oxides; In vivo; Zebrafish embryos; Cytotoxicity; Endodontics; Bioceramic root canal sealers
• ISSN: 2008-210X; 2008-2118
• DOI: 10.34172/joddd.2023.39163

This study evaluated the cytotoxicity of four bioceramic root canal sealers (RCSs) in vivo. The embryonic zebrafish characteristics, such as mortality, survival, hatching, and general morphology, served as the parameters for assessing cytotoxicity. The RCSs, namely GuttaFlow Bioseal, MTA Fillapex, CeraSeal Bioceramic, and iRoot SP, were mixed according to the manufacturer's guidelines. The extract solution was prepared by immersing the set RCS into 1X dilution of E3 solution. Then, the extract solution was delivered into a Petri dish where zebrafish embryos were allowed to develop. Cytotoxicity was evaluated 24, 48, 72, and 96 hours after fertilization. The Kruskal-Wallis test showed that except for GuttaFlow Bioseal, the mortality, survival, and hatching of zebrafish embryos for the remaining three bioceramic RCSs were significantly different from the negative controls ( <0.05). Significant differences were also evident in the mortality, survival, and hatching of zebrafish embryos between GuttaFlow Bioseal and three other RCSs ( <0.05). GuttaFlow Bioseal was less cytotoxic than other bioceramics RCSs; MTA Fillapex, CeraSeal Bioceramic root canal sealer, and iRoot SP root canal sealer exhibited comparable cytotoxicity.